5-杂氮-2′-脱氧胞苷对鼻咽癌细胞增殖、凋亡和周期的影响  

Influence of 5-Aza-2′-deoxycytidine on the Proliferation,Apoptosis and Cycle of Nasopharyngeal Carcinoma Cell Lines

作　　者：谭双香[1] 易红[1] 汤参娥[1] 陈主初[1] 肖志强[1] 

机构地区：[1]中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,湖南长沙410008

出　　处：《南华大学学报（医学版）》2008年第4期445-448,共4页Journal of Nanhua University(Medical Edition)

摘　　要：目的分析5-杂氮-2′-脱氧胞苷(5-aza-2dC)对鼻咽癌细胞株CNE1、CNE2、5-8F、6-10B增殖、凋亡和细胞周期分布的影响。方法用不同浓度5-aza-2dC处理鼻咽癌细胞,MTT实验观察细胞生长曲线,用流式细胞仪检测细胞周期分布情况和凋亡细胞比例。结果5-aza-2dC对4株细胞的增殖均具有抑制作用,CNE1和6-10B细胞的半数抑制剂量低于CNE2和5-8F。药物处理使4株细胞凋亡率呈剂量依赖性增加,CNE1和6-10B细胞对药物敏感性高于CNE2和5-8F。经5-aza-2dC处理后,CNE1、CNE2、6-10B细胞出现不同程度G0/G1期阻滞,而5-8F表现为G0/G1期和G2/M期双期阻滞,CNE1和6-10B细胞对药物敏感性高于CNE2和5-8F。结论5-aza-2dC对CNE1、CNE2、5-8F和6-10B细胞具有抑制增殖、促进凋亡和细胞周期阻滞作用,CNE1和6-10B对药物敏感性高于CNE2和5-8F。Objective To explore the influence of 5-aza-2＇-deoxycytidine（5-aza-2dC）on the proliferation, apoptosis and cell cycle distribution of four nasopharyngeal carcinoma cell lines-CNE1, CNE2,5-8F and 6-l0B. Methods Nasopharyngeal carcinoma cell lines were treated with different concentrations of 5-aza-2dC. Then MTT assay was used to detect the growth curves of cells. Flow cytometry was adopted to discover cell cycle distribution and apoptosis cells. Results Proliferation of CNE1, CNE2,5-8F,6-10B were all inhibited by 5 - aza- 2dC treatment,while the 50% inhibiting concentration to CNE1 and 6-10B were lower than to CNE2 and 5-8F. The apoptosis cells were dose-dependently increased after 5-aza-2dC treatment in all four cell lines; CNE1 and 6-10B were more sensitive than CNE2 and 5-8F. CNE1, CNE2. and 6 - 10B blocked in G0/G1 phase while 5-8F blocked in G0/G1 phase and G2/M phase after 5-aza-2dC treatment, CNE1 and 6-10B were more sensitive than CNE2 and 5 - 8F. Conclusion To nasopharyngeal carcinoma cell lines of CNE1, CNE2,5-8F and 6-10B,5-aza-2dC can inhibit proliferation, promote apoptosis,and block cell cycle,CNE1 and 6-10B were more sensitive than CNE2 and 5-8F.

关 键 词：5-杂氮-2’-脱氧胞苷 鼻咽肿瘤 细胞周期 增殖 凋亡 

分 类 号：R739.6[医药卫生—肿瘤]