声门癌中S期激酶相关蛋白2和PTEN蛋白的表达及其相关性研究  

Expression of Skp2 and PTEN and its correlation in glottic carcinoma

作　　者：赵洪春[1] 何晓光[2] 蒲章杰[1] 王秋芹[1] 

机构地区：[1]滨州医学院附属医院耳鼻咽喉头颈外科,山东滨州256603 [2]昆明医学院第一附属医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2008年第15期688-691,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:检测声门癌中S期激酶相关蛋白2(Skp2)和PTEN蛋白表达,探讨它们的临床意义及相关性。方法:采用免疫组织化学方法检测Skp2、PTEN蛋白在42例声门癌、16例声带不典型增生和27例声带息肉组织中的表达,并进行统计分析。结果:在声带息肉、声带不典型增生和声门癌组织中Skp2蛋白的阳性率分别为11.11%、37.50%、40.48%,各组间均差异有统计学意义(均P<0.01)。PTEN蛋白在各组病变中的阳性率分别为100.00%、75.00%、52.38%,各组间均差异有统计学意义(均P<0.05);Skp2蛋白和PTEN蛋白与声门癌的临床分期、颈部淋巴结转移、预后有关(均P<0.05)。在声门癌组织中Skp2和PTEN蛋白的表达呈负相关关系,相关系数r=-0.4301(P<0.01)。结论:Skp2和PTEN蛋白表达与声门癌的发生、转移和预后等生物学行为有关,Skp2可能是声门癌癌变的早期分子事件。在声门癌中Skp2蛋白高表达与PTEN蛋白的低表达呈负相关。Objective：To investigate the expression and clinical siginificance of Skp2 and PTEN in glottic carcinoma and the relationship between the two genes. Method： Formalin-fixed and paraffin-embedded tissues, which came from 42 cases of glottic carcinoma and 16 cases of atypical hyperplasia of vocal fold and 27 cases of vocal cord polyp, were detected for the expression of Skp2, PTEN by SP immunohistochemistry, then we analyzed the result statistically. Result：The expression rates of Skp2 protein in vocal cord polyp, atypical hyperplasia of vocal cord and glottie carcinoma were 11.11%, 37.50%, 40.48% respectively. There was significant difference among them （P〈0.01） ; the expression rates of PTEN protein in vocal cord polyp, atypical hyperplasia of vocal cord and glottic carcinoma were 100. 00%, 75.00%, 52.38% respectively. There was significant difference among them （P〈0.05） ; the expressions of Skp2 and PTEN in glottic carcinoma were associated with clinical stage, lymph nodal metastases and prognosis （P〈0.05） ; there was a negative correlation between the expression of Skp2 and PTEN, and their correlation coefficient was r=-0.4 301 （P〈0.01）. Conclusion：The expressions of Skp2 and PTEN may play an important roles in the tumorigenesis, metastases and poor prognosis of glottie carcinoma. These changes may be the early molecular event of the carcinogenesis. The high expression of Skp2 was negative correlation with the lower PTEN in glottic carcinoma.

关 键 词：声门肿瘤 S期激酶相关蛋白 PTEN蛋白 免疫组织化学 

分 类 号：R739.6[医药卫生—肿瘤]