银莲花素A对S180、H22和U14细胞小鼠移植瘤的抑制作用  

Antitumor Effects of Raddeanin A on S180,H22 and U14 Cell Xenografts in Mice

作　　者：王明奎[1] 丁立生[1] 吴凤锷[1] 

机构地区：[1]中国科学院成都生物研究所天然产物研究中心,四川成都610041

出　　处：《癌症》2008年第9期910-913,共4页Chinese Journal of Cancer

摘　　要：背景与目的:有研究证明,从两头尖中分离得到的三萜皂甙银莲花素A在体外具有较好的抑瘤活性。本研究将观察银莲花素A在体内对小鼠移植瘤的抑制活性。方法:MTT法检测银莲花素A对人鼻咽癌KB细胞和人卵巢癌SKOV3细胞的抑制率。体内实验选取小鼠肉瘤S180细胞、小鼠肝癌H22细胞和小鼠宫颈癌U14细胞,观察银莲花素A注射给药对S180、H22和U14细胞小鼠移植瘤的抑瘤率,以及灌胃给药对小鼠肉瘤S180移植瘤的抑瘤率。测定银莲花素A的急性毒性。结果:银莲花素A在体外对KB细胞和SKOV3细胞的IC50分别为4.64!g/mL和1.40!g/mL。4.5mg/kg银莲花素A腹腔注射对S180、H22和U14细胞小鼠移植瘤的抑瘤率分别为60.5%、36.2%和61.8%。200mg/kg灌胃给药时抑瘤率为64.7%。灌胃给药和腹腔注射银莲花素A的LD50分别为1.1g/kg和16.1mg/kg。结论:银莲花素A在体内外均具有较好的抑瘤作用,体内可抑制小鼠移植瘤的生长,是一个有潜力的抗癌药物。BACKGROUND ＆ OBJECTIVE： Raddeanin A, a triterpenoid saponin from Anemone raddeana Regel, has good antitumor activity in vitro. This study was to investigate its antitumor effects on tumor cell xenografts in mice. METHODS： The inhibitory effects of raddeanin A on the proliferation of human nasopharyngeal carcinoma KB cells and ovarian cancer SKOV3 cells were measured by MTT assay. The inhibitory effects of raddeanin A injection on the growth of sarcoma S180, liver cancer H22 and cervical carcinoma U14 cell xenografts in mice and the effect of raddeanin A lavage on the growth of S180 cell xenografts were measured. The acute toxicity of raddeanin A was also measured. RESULTS： The 50% inhibition concentration （IC50） of raddeanin A was 4.64 ug/mL for KB cells and 1.40 ug/mL for SKOV3 cells. When injected with raddeanin A at a dose of 4.5 mg/ kg, the growth inhibition rates of S180, H22 and U14 cell xenografts were 60.5%, 36.2% and 61.8%, respectively. When lavaged with raddeanin A at a dose of 200 mg/kg, the growth inhibition rate of S180 cell xenografts was 64.7%. The median lethal dose （LD50） of raddeanin A lavage was 1.1 g/kg and that of raddeanin A injection was 16.1 mg/kg. CONCLUSION： Raddeanin A has good antitumor activity both in vitro and in vivo, and would be a potential antitumor medicine.

关 键 词：两头尖 银莲花素A 抑瘤活性 S180细胞 H22细胞 U14细胞 小鼠 移植瘤 

分 类 号：R73-36[医药卫生—肿瘤]