转染α1,2-岩藻糖转移酶基因对人卵巢癌细胞系RMG-1癌相关基因表达的影响  

Impact of α1,2-Fucosyl Transferase Gene Transfection on Cancer-related Gene Expression Profile of Human Ovarian Cancer Cell Line RMG-1

作　　者：朱连成[1] 林蓓[1] 郝莹莹[1] 李飞飞[1] 刁斌[2] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]沈阳市第四人民医院妇产科,辽宁沈阳110031

出　　处：《癌症》2008年第9期934-941,共8页Chinese Journal of Cancer

基　　金：国家自然基金项目(No.30170980;No.30571958);教育部归国人员博士启动基金项目(No.2000479);辽宁省博士启动基金项目(No.001040);辽宁省自然基金项目(No.20052107);辽宁省教育厅基金项目(No.05L492)~~

摘　　要：背景与目的:前期研究证明转染外来α1,2-岩藻糖转移酶(α1,2-fucosyl transferase,α1,2-FT)基因后人卵巢癌细胞系RMG-1的恶性生物学行为加强。本研究的目的是探讨转染α1,2-FT基因对卵巢癌细胞系RMG-1癌相关基因表达的影响。方法:将基因表达载体pcDNA3.1-HFT-H和空载体pcDNA3.1转染至RMG-1细胞中分别生成RMG-1-H细胞和RMG-1-C细胞,利用基因芯片对这两种细胞的基因表达序列进行分析,使用GoMiner在线数据库进行信息查询。结果:与RMG-1细胞比较,RMG-1-H细胞中有88种差异性表达基因,其中60种基因表达增强,28种基因表达降低。检索其基因功能,发现在分子功能、生物学行为和细胞成分定位三个分支上,差异表达的基因参与到了诸如蛋白质结合、核苷酸结合,细胞增殖、DNA依赖性转录的调节、信号转导、蛋白氨基酸磷酸化、转录、细胞粘附等多方面。结论:转染α1,2-FT基因使卵巢癌RMG-1细胞的基因表达发生了改变。BACKGROUND ＆ OBJECTIVE. The malignant biological behaviors are enhanced in human ovarian cancer cell line RMG-1 after transfection of α1,2-fucosyl transferase α1,2-FT） gene. This study was to investigate the influence of α1 ,2-FT gene transfection on cancer-related gene expression profile of RMG-1 cells. METHODS; Gene expressing vector pcDNA3.1-HFT-H and empty vector pcDNA3.1 were transfected into RMG-1 cells to produce RMG-1-H and RMG-1-C cells separately. Gene expression profiles of these two cell lines were detected by gene chip assay, The acquired data were inquired on the GoMiner online database. RESULTS： After transfection, comparing with those in RMG-1-C cells, 88 differentially expressed genes were identified in RMG-1-H cells. 60 were up-regulated and 28 were down-regulated. These genes are involved in protein binding, nucleotide binding, cell proliferation, DNA-dependent regulation of transcription, signal transduction, protein amino acid phosphorylation, transcription, cell adhesion, and so on. CONCLUSION; The transfection of α1,2-FT gene causes the changes of gene expression profile in ovarian cancer RMG-1 cells.

关 键 词：卵巢肿瘤 RMG-1-H细胞 基因表达谱 寡核苷酸芯片 α1 2-岩藻糖转移酶基因 

分 类 号：R737.31[医药卫生—肿瘤]