Polo样激酶1在大鼠心肌细胞分化过程中的表达  

EXPRESSION OF Polo-LIKE KINASE 1 DURING THE DIFFERENTIATION OF CARDIOMYOCYTES IN RATS

作　　者：邸菁[1] 柏树令[1] 张凌志[2] 高杰[1] 佟浩[1] 

机构地区：[1]中国医科大学基础医学院人体解剖学教研室,沈阳110001 [2]中国医科大学附属盛京医院急诊科,沈阳110004

出　　处：《解剖学报》2008年第4期578-581,共4页Acta Anatomica Sinica

基　　金：辽宁省教育厅资助项目(051525)

摘　　要：目的通过检测polo样激酶1(plk1)在大鼠生后不同发育阶段心肌组织中的表达,探讨心肌细胞退出细胞周期的相关机制。方法生后0d、3d、7d、10d、2周和成年健康Wistar大鼠各5只,采用针对横纹肌肌动蛋白和磷酸化组蛋白H3的免疫荧光双标方法,测定各组大鼠心肌细胞有丝分裂指数;用RT-PCR和免疫印迹技术,检测心肌组织plk1 mRNA和蛋白表达的改变。结果大鼠生后0d心肌细胞有丝分裂指数[(0.905±0.087)%]是生后2周[(0.372±0.094)%]的2.4倍(P<0.01);plk1基因和蛋白在大鼠生后表达明显下调,2周后未检测到表达。结论plk1基因的表达下调与大鼠心肌细胞增殖停滞密切相关,在大鼠心肌细胞退出细胞周期的调控机制中发挥重要作用。Objective To understand the mechanism of how cardiomyocytes exit from the cell cycle, we examined the expression of polo-like kinase 1 （plkl） in the postnatal developmental process of cardiac myocytes. Methods Mitotic Index（MI） of cardiomyocytes was examined in the neonatal, 2-week-old, 4-week-old, and adult rat hearts （five cases per groups） by double immunofluorescence stained with H3P and a-sarcomeric actin antibodies, plkl mRNA and protein expression during the postnatal developmental process of cardiac myocytes were detected by RT-PCR and Western blot analysis in rat hearts. Results The MI of cardiomyocytes in 0-day-old hearts （0.905 ± 0.087% ） was approximately 2.4 times over that in 2-week-old hearts（0.372 ± 0.094% ）（ P 〈 0.01）. The mRNA expression of plkl is obviously declined during postnatal 7 days to 10 days in rat hearts（ P 〈 0.01）, and was undetectable in the adult group. Meanwhile, the protein level of PLK was down-regulated after birth, especially during the 0-day-old to 7-day-old（ P 〈 0.01 ）period and 10-day-old to 2-week-old （ P 〈 0.01 ） period in rat hearts. Conclusion Down-regulation of plkl correlates well with the loss of proliferative ability of cardiac myocytes and that plkl is shown to be important for the permanent withdrawal of cardiomyocytes from the cell cycle.

关 键 词：POLO样激酶1 心肌细胞 细胞周期调控 RT-PCR 免疫印迹法 免疫荧光 大鼠 

分 类 号：R-332[医药卫生] R36