超声辐照对神经母细胞瘤株化疗敏感性的影响  

Effect of ultrasound on the chemotherapeutic sensitivity of Neuroblastoma cells

作　　者：李勤[1] 汪洋[2] 金先庆[1] 刘伟[1] 赵珍珍[1] 

机构地区：[1]重庆医科大学附属儿童医院普外科,400014 [2]重庆医科大学卫生毒理教研室

出　　处：《中华小儿外科杂志》2008年第8期467-470,共4页Chinese Journal of Pediatric Surgery

基　　金：国家自然科学基金重点项目（30330590）

摘　　要：目的研究超声辐照对神经母细胞瘤株SK-N-SH细胞化疗敏感性的影响。方法将SK-N-SH细胞分为长春新碱组（VCR组）和长春新碱＋超声组（VCR＋U组）。VCR组细胞加入不同浓度的长春新碱，进行超声假照。VCR＋U组细胞在加入不同浓度的长春新碱后再接受超声辐照（频率：0．8MHz，声强：1．0W／cm^2，时间：40s），用MTT法检测各组细胞的存活率。结果当VCR浓度较低时，VCR＋U组细胞存活率较VCR组明显降低。结论超声辐照可以增强SK-N-SH细胞对化疗药物长春新碱的敏感性。Objective To research the effect of ultrasound on the chemotherapeutic sensitivity of SK-N-SH Neuroblastoma cells. Methods The SK-N-SH cells were divided into 2 groups： the ex- perimental and the control, The experimental group were then divided into VCR and VCR ＋ Ultra- sound （U） subgroups. In VCR group, the cell suspensions were treated with sham irradiation after adding VCR with different concentration; in VCR ＋ U group, the cell suspensions were treated with ultrasound irradiation after adding VCR with different concentration. MTT assay was used to assess the survival rate of SK-N-SH cells in each group. Results The survival rate of SK-N-SH cells in VCR ＋ U group was lower than that in VCR group when the concentrations of VCR were relatively low. Conclusions Ultrasound can increase the cytotoxic effect of VCR on SK-N-SH cells.

关 键 词：超声疗法 神经母细胞瘤 长春新碱 

分 类 号：R739.4[医药卫生—肿瘤]