不同分化程度的鼻咽癌细胞系质膜差异蛋白质组分析(英文)  

Comparative Proteome Analysis of Plasma Membrane from Different Differential Nasopharyngeal Carcinoma Cell Lines

作　　者：盛婷婷[1] 张丽军[1] 刘晓慧[1] 贾小芳[1] 熊继先[1] 贺权源[1] 曹锐[1] 彭霞[1] 石妮[1] 梁宋平[1] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学实验室,长沙410081

出　　处：《中国生物化学与分子生物学报》2008年第8期712-718,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：Supported by Major State Basic Research Development Programof China (973Program,No.2001 CB5102)~~

摘　　要：本研究以CNE1和CNE2为材料,采用亚细胞蛋白质组研究方法研究不同分化程度鼻咽癌细胞系的差异蛋白质.首先用Percoll密度梯度离心法获得高纯度质膜,通过双向凝胶电泳分离、PDQuest软件分析后找出在肿瘤细胞中表达变化的蛋白质点,再用基质辅助激光解析电离飞行时间串联质谱(MALDI-TOF/TOF-MS)进行鉴定,共鉴定到9个具有2倍或2倍以上差异的蛋白质.这些表达差异的蛋白质参与了细胞分化、代谢及细胞信号传导过程.我们对其中5个蛋白质进行了实时定量PCR分析,对其中4个蛋白质的表达进行了免疫印迹验证.本试验为研究不同分化程度的鼻咽癌提供了一种蛋白质组研究方法,并且找到了galectin-1、annexinⅡ等一些可能与分化相关的蛋白质.这些数据对于研究鼻咽癌的生物学特性具有非常重要的意义.A subcellular proteomic method was applied to investigate the protein expression profiles of nasopharyngeal carcinoma （NPC） cell lines, CNE1 and CNE2, at various differentiation levels. Plasma membrane （PM） proteins were obtained by Percoll density grade centrifugation and subjected to twodimensional electrophoresis （2DE） followed by PDQuest software analysis. Nine proteins expressed with more than two folds difference were identified by MALDI-TOF-TOF, of which functions involved in cell differentiation, signal transduction, and metabolism. Half of these proteins, such as galectin-1 and annexin Ⅱ , were analyzed with real-time quantitative PCR or Western blotting. We have tested a proteomic method to study differentiated NPCs at different levels and found several proteins that might be related to their biological characteristics.

关 键 词：鼻咽癌 分化 质膜 质谱 蛋白质组 

分 类 号：Q915.813.3[天文地球—古生物学与地层学] S512.101[生物学—古生物学]