一种复方制剂对尾部悬吊大鼠比目鱼肌mATP酶活性升高及肌萎缩的对抗效应观察  

THE PREVENTIVE EFFECTS OF ONE HERBAL COMPOUND ON ACTIVITIES OF MYOSIN ADENOSINE TRIPHOSPHATASE OF MUSCLE FIBERS AND MUSCLE ATROPHY IN TAIL-SUSPENDED RAT

作　　者：张海祥[1] 何志仙[2] 高云芳[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西安建筑科技大学理学院,陕西西安710055

出　　处：《中国应用生理学杂志》2008年第3期367-372,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30300106);西北大学自然科学基金(04NW39)

摘　　要：目的:探讨一种中药复方制剂RC对模拟失重条件下大鼠比目鱼肌(SOL)的mATP酶活性及肌萎缩的影响。方法:尾部悬吊法建立大鼠模拟失重模型,测量比目鱼肌的湿重体重比(mg/g),以Ca2+-ATPase法测定比目鱼肌中mATP酶活性,计算Ⅰ型和Ⅱ型肌纤维的比例,测定比目鱼肌中Ⅰ型和Ⅱ型肌纤维的横截面积(CSA)。结果:与吊尾+溶媒灌胃组相比,吊尾14天后,吊尾+RC灌胃组的湿重体重比增加了33.33%(P<0.01);比目鱼肌中Ⅰ型和Ⅱ型肌纤维CSA均显著增加(143.03%,P<0.01;83.25%,P<0.01);比目鱼肌中Ⅰ型肌纤维比例明显升高,Ⅱ型肌纤维比例明显降低(P<0.01)。结论:复方制剂RC对模拟失重条件下大鼠比目鱼肌mATP酶活性的升高与模拟失重引起的肌萎缩有显著的对抗作用。Aim： To study the effect of radix-astragali compound（RC） on muscle atrophy in tail-suspended rats. Muscle weight, fiber type distribution, cross-sectional area（CSA）, and activity of myosin adenosine triphosphatase（ATPase） in rat soleus muscle were investigated. Methods： The tail-suspended rats were subjected to a 14 days simulated weightlessness, during which period, RC or saltwater was given via intragastric instillation during tail suspension. The changes of soleus muscle weight were scaled by muscle-to-body weight ratio. The activities of myosin ATPase of muscle fibers were detected by method of Ca^2＋ -ATpase. Results： After a 14 days tail suspension it was found： in rats treated with RC, soleus muscle-to-body weight ratio rose by 33.33 % （P 〈 0.01 ）, both CSA of type 1 and II fiber drastically enhanced by（ 143.03 % , P 〈 0.01 ; 83.25 % , P 〈 0.01 ）, the percentage of type 1 fiber significantly declined compared to the untreated rats. Conclusion： RC is able to effectively prevent muscle atrophy caused by tail suspension and restrain the increase in the myosin ATPase activities caused by simulated weightlessness.

关 键 词：模拟失重 比目鱼肌 肌球蛋白ATP酶 肌萎缩 

分 类 号：R337[医药卫生—人体生理学]