幽门螺旋杆菌感染对帕金森病患者血清氧化应激状态及血小板线粒体功能的影响  

Influence on Plasma Oxidative Stress State and Platelet Mitochondria Function in Sporadic Parkinson Disease with Helicobacter Pylori Infection

作　　者：罗晨[1] 肖波[1] 刘人恺[1] 杨晓苏[1] 吴志国[1] 龙莉莉[1] 冯莉[1] 

机构地区：[1]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《中国神经免疫学和神经病学杂志》2008年第5期342-345,348,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：高等学校博士学科点专项科研基金(20030533042)

摘　　要：目的通过观察幽门螺旋杆菌(HP)感染对帕金森病(PD)患者血清脂质过氧化物、抗氧化物水平及血小板线粒体ComplexⅠ活性的影响,探讨HP感染导致PD发生的可能机制。方法采用回顾性研究,以53例PD患者和50例年龄、性别相匹配的非PD患者为研究对象,利用Western-blot技术检测血清中HP-IgG抗体类型、分光光度法检测血清丙二醛(MDA)、还原型谷胱甘肽(GSH)水平及血小板线粒体ComplexⅠ活性。结果(1)PD组HP抗体阳性率及Ⅰ型感染比例均明显高于对照组(P<0.05)。(2)血小板线粒体ComplexⅠ活性PD组较对照组显著下降(P<0.01),PD(HP+)组较对照(HP-)组显著下降(P<0.05)。(3)血清MDA水平PD组与对照组比较差异无统计学意义(P>0.05),但PD(HP+)组较对照(HP-)组显著增高(P<0.05)。(4)血清GSH水平PD组与对照组比较差异无统计学意义(P>0.05),PD(HP+)组、PD(HP-)组、对照(HP+)组及对照(HP-)组两两比较差异均无统计学意义(均P>0.05)。(5)PD患者血小板线粒体ComplexⅠ活性与UPDRS评分呈负相关;血清MDA水平与年龄、UPDRS评分、病程均无相关性;血清GSH水平与病程呈负相关。结论HP感染导致的系统性氧化应激状态及线粒体功能损伤可能参与了PD的发生。Objective To analyze the influence of Helicobacter Pylori （HP） infection on the plasma oxidative stress state and platelet mitochondrial function in patients suffered Parkinson disease （PD） and to determine the role of HP infection in the pathogenesis of PD. Methods Fifty-three patients with PD and fifty age-matched control subjects were analyzed. Plasma IgG against HP were measured by western-blot. Plasma MDA, GSH level and platelet mitochondria complex activity Ⅰ were measured by spectrophotometry. Results The seropositivity for HP antibody and type Ⅰ infection were significantly higher in PD group than in control group （P〈0.05）. Platelet mitochondria complex Ⅰ activity： there were significant differences between PD group and control group （P〈0.05）, PD（HP^＋） group and control（HP^- ） group （P〈0. 057. Plasma MDA content： there were no significant difference between PD group and control group （P〉0.05）, but significantly different between PD （HP^＋） group and control （HP^- ） group （P〈0.05）. Plasma GSH content： there were no significant difference between PD group and control group （P〉0.05） or among PD （HP^＋）, PD（HP^-）, control （HP^＋）. and control （HP^-） groups （P〉0.05）. The platelet mitochondria complex Ⅰ activity was negatively correlated with UPDRS scores （P〈0.05） and there was no correlation between complex Ⅰ activity and age or duration, plasma MDA and UPDRS scores, age or duration. The plasma GSH was negatively correlated with duration （P〈0.05）, and there were no correlation between plasma GSH and UPDRS scores or age. Conclusions HP infection caused systemic oxidative stress and mitochondria dysfunction in definite tissues, that might participated in the pathogenesis of PD.

关 键 词：帕金森病 幽门螺旋杆菌 氧化应激 线粒体功能障碍 

分 类 号：R741[医药卫生—神经病学与精神病学]