VEGF的表达与大肠癌组织生长、侵袭及转移的关系  

Expression of vascular endothelial growth factor expression and its relation with growth,invasion and metastasis of human colorectal cancer

作　　者：朱云松[1] 杨大明[2] 姚登福[2] 钱俊波[1] 李玉明[1] 许利华[1] 

机构地区：[1]南通医学院第二附属医院消化内科,江苏省南通市226001 [2]南通医学院附属医院,江苏省南通市226001

出　　处：《世界华人消化杂志》2008年第22期2549-2552,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨血管内皮生长因子表达与大肠癌组织的生长、侵袭和转移的关系.方法:手术切除新鲜大肠癌组织、远癌组织标本各23份,结肠息肉16例,另外收集结肠癌患者45例.应用逆转录聚合酶链(RT-PCR)和ELISA法,对大肠癌组织和血清VEGF进行测定,并分析VEGF与大肠癌Dukes临床病理分期的关系.结果:23例大肠癌组血管内皮生长因子mRNA阳性率明显高于远癌组及结肠息肉组(65.22%vs21.74%,18.75%,均P<0.01).根据Dukes临床病理分期,A和B期VEGF mRNA阳性为3例(37.5%),C与D期阳性有12例(80.0%),两者差异有显著性(P<0.05).45例结肠癌C和D期血清VEGF水平明显高于A和B期(716.71+229.39ng/Lvs312.30+103.61ng/L),两者差异有显著性(P<0.01).结论:血管内皮生长因子可作为大肠癌生长、侵袭和转移的临床预后判断指标.AIM： To explore the relations of vascular endothelial growth factor （VEGF） expression with the growth, invasion and metastasis of colorectal cancer. METHODS： We collected 23 fresh colorectal cancerous, distal cancerous tissues resected during operation, 16 colon polyps and 45 serum samples from colon cancer patients. The expression of VEGF in colorectal tissues and serum samples was detected by nested reverse transcription polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbent assay （ELISA）, respectively. The relationship was also analyzed between VEGF expression and Duke＇s staging RESULTS： The expression of VEGF mRNA was significantly higher in the colorectal cancer tissues than that in the distal cancerous tissues or colon polyps （65.22% vs 21.74%, 18.75%, both P 〈 0.01）. VEGF mRNA expression was positive in 3 patients （37.5%） with Duke＇s stage A or B, and it was positive in 12 patients with （80.0%） stage C or D; there was significant difference between them （P 〈 0.05）. The level of serum VEGF was markedly higher in patients with stage C or D than that in patients with stage A or B （716.71 ± 229.39 ng/L vs 312.30 ± 103.61 ng/L, P 〈 0. 01）. CONCLUSION： VEGF can serve as an index in predicting the growth, invasion and metastasis of colorectal cancer.

关 键 词：大肠癌 血管内皮生长因子 生长 侵袭 转移 

分 类 号：R735.34[医药卫生—肿瘤]