N-乙酰半胱氨酸对C3A永生化肝细胞解毒代谢功能的影响  

Effects of N-acetylcysteine on the detoxif ication and biological metabolism of C3A immortalized cell line

作　　者：魏琳琳[1] 郑素军[1] 陈煜[1] 赵军[1] 丁美[1] 段钟平[1] 

机构地区：[1]首都医科大学附属北京佑安医院人工肝中心,北京市100069

出　　处：《世界华人消化杂志》2008年第23期2610-2614,共5页World Chinese Journal of Digestology

基　　金：国家科技攻关计划引导项目;No.2003BA753C;2007年高等学校博士学科点专项科研基金;No.20070025009~~

摘　　要：目的:研究N-乙酰半胱氨酸(N-acetylcysteine,NAC)对慢性重型肝炎肝衰竭时肝细胞解毒代谢功能的影响,并比较NAC与还原型谷胱甘肽(GSH)的疗效.方法:体外慢性重型肝炎患者血浆(chronic severe hepatitis plasma,CSHP)培养C3A细胞,并同时分别加入大、中、小三个剂量NAC及对应剂量的GSH,以正常血浆(normal human plasma,NHP)及培养基(100 mL/L FBS-MEM)为对照,分别于24、48、72h3个时间点检测细胞内GSH及脂质过氧化物(MDA)的含量,并检测安定代谢量的变化.结果:24、48、72h3个时间点,培养基组、正常血浆组、各剂量NAC治疗组和GSH治疗组C3A细胞中GSH含量与安定代谢量均分别高于慢性重型肝炎血浆组(GSH:F=246.116,235.489,201.536,均P<0.01;安定代谢量:F=306.812,476.722,502.061,均P<0.01),而慢性重型肝炎血浆组C3A细胞中MDA含量均分别高于上述各组(F=332.48,662.349,492.983,均P<0.01).慢性重型肝炎血浆+NAC组与慢性重型肝炎血浆+GSH组比较,前者细胞内的GSH含量和对安定的代谢量,大、中、小剂量组均分别高于后者对应剂量组(P<0.01),而前者细胞内的MDA含量,大、中、小剂量组均分别低于后者对应剂量组(P<0.01).结论:NAC可以显著改善慢性重型肝炎肝衰竭时肝细胞解毒代谢功能,GSH也有相似功效,且相同摩尔剂量的NAC效果要优于GSH.AIM： To investigate the effects of N-acetylcysteine （NAC） on hepatocyte （using C3A immortalized cell line） detoxification and biological metabolism, and to compare the therapeutic effect between NAC and GSH （reduced glutathione）. METHODS： C3A cells were cultured with chronic severe hepatitis plasma （CSHP）, while NAC and GSH were added to the medium with a dosage of 25 mmol/L, 5 mmol/L or 1 mmol/L, respectively. Meanwhile, C3A cells cultured with normal human plasma （NHP） or 100 mL/L FBS-MEM were as controls. The contents of GSH and MDA in C3A cells and the metabolic rate of diazepam were detected 24, 48 and 72 h after administration, respectively. RESULTS： Comparison between groups showed that the content of intra-cellular GSH and the metabolic rate of diazepam in C3A cells were significantly higher at 24, 48 and 72 h in the FBS-MEM group, the NHP group and the groups added NAC or GSH in CSHP than those in the CSHP group （GSH： F = 246.116, 235.489, 201.536, all P 〈 0.01; diazepam： F = 306.812, 476.722, 502.061, all P 〈 0.01）, but the content of intra-celluar MDA in the CSHP group was markedly higer than those in the other groups （F = 332.48, 662.349, 492.983, all P 〈 0.01）. The content of intra-celluar GSH and the metabolic rate of diazepam in C3A cells in the CSHP ＋ NAC groups were higher than those in the CSHP ＋ GSH groups （comparison between the same dosage： P 〈 0.01）, but the content of intra-celluar MDA was in the opposite situation （P 〈 0.01）. CONCLUSION： NAC can obviously improve the detoxification and biological metabolism of C3A cells cultured in CSHP. NAC is superior to GSH at the same dosage.

关 键 词：C3A细胞 N-乙酰半胱氨酸 慢性重型肝炎 解毒 代谢 

分 类 号：R575.1[医药卫生—消化系统]