诱导型一氧化氮合酶和血红素氧合酶-1在反流性食管炎患者食管黏膜中的表达  

Expression of inducible nitric oxide synthase and heme oxygenase-1 in the esophageal mucosa of patients with reflux esophagitis

作　　者：李军杰[1] 方红丽[2] 王月香[1] 郑勇[3] 孙侃[3] 常向云[3] 陈卫刚[3] 王晓丽[3] 赵瑾[4] 

机构地区：[1]新疆石河子大学医学院第一附属医院老干一科,新疆维吾尔自治区石河子市832002 [2]新疆石河子大学医学院,新疆维吾尔自治区石河子市832008 [3]新疆石河子大学医学院第一附属医院消化内科,新疆维吾尔自治区石河子市832002 [4]新疆石河子大学医学院病理科,新疆维吾尔自治区石河子市832008

出　　处：《世界华人消化杂志》2008年第24期2713-2718,共6页World Chinese Journal of Digestology

摘　　要：目的:研究诱导型一氧化氮合酶(iNOS)和血红素氧合酶(HO-1)在反流性食管炎(RE)患者食管黏膜中的表达及两者之间的关系,以探讨气体信使分子NO和CO在RE的发病机制中的作用.方法:以免疫组化SP法结合诱导型一氧化氮合酶(iNOS)和血红素氧合酶(HO-1)的免疫反应阳性产物的平均吸光度图像分析法检测77例RE患者(分4级组)及20例对照组的食管黏膜中iNOS和HO-1蛋白的表达强度,并分析两者的关系.结果:iNOS和HO-1主要在食管黏膜上皮细胞中表达,RE患者食管黏膜中iNOS与HO-1的表达明显强于对照组(0.2473±0.0548vs0.1616±0.0169,0.2334±0.0511vs0.1776±0.0164,均P<0.01).且RE组食管黏膜上皮细胞iNOS和HO-1蛋白表达呈显著正相关(r=0.513,P=0.000);而正常组中iNOS和HO-1蛋白表达之间无相关性(r=-0.073,P=0.758).结论:RE患者食管黏膜中iNOS和HO-1表达异常,表明NO和CO在RE的发病机制中可能起重要作用.AIM： To detect the expression of inducible nitric oxide synthase （iNOS） and heme oxygenase-1 （HO-1） in human esophageal mucosa of patients with reflux esophagitis （RE）, and to find out the role of gas messenger molecules such as nitric oxide （NO） and carbon monoxide （CO） in the pathogenesis of RE. METHODS： The distributions of iNOS and HO-1 proteins in esophageal tissues 77 RE patients and 20 normal controls were detected by immunohistochemistry （SP method）, and the relationship between iNOS and HO-1 expression was analyzed. RESULTS： iNOS and HO-1 were expressed mainly in esophageal mucosal epithelia. The expression levels of iNOS and HO-1 in RE patients were significantly higher than those in the normal controls （0.2473 ± 0.0548 vs 0.1616 ± 0.0169; 0.2334 ± 0.0511 vs 0.1776 ± 0.0164; both P 〈 0.01）. Moreover the expression of HO-1 was positively correlated with iNOS expression in esophageal mucosa of RE patients （r = 0. 513, P = 0.000）. There was no marked difference between iNOS and NO expression in the normal controls （r = -0.073, P = 0.758）. CONCLUSION： HO-1 and iNOS are abnormally expressed in esophageal mucosa of RE patients, suggesting that NO and CO may play important roles in the pathogenesis of gastrointestinal motility disorders such as RE.

关 键 词：反流性食管炎 诱导型一氧化氮合酶 血红素氧合酶-1 免疫组织化学 一氧化氮 一氧化碳 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R562.25[医药卫生—临床医学]