苦参碱对前列腺癌细胞增殖及雄激素受体功能的抑制作用  

Matrine Inhibits the Proliferation of Prostate Cancer Cells and the Activity of Androgen Receptor

作　　者：谌科[1] 胡志全[1] 王涛[1] 郭峻莉[2] 郭辉[1] 叶章群[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430030 [2]华中科技大学同济医学院病理教研室,湖北武汉430030

出　　处：《中华男科学杂志》2008年第8期719-722,共4页National Journal of Andrology

摘　　要：目的:探讨苦参碱(matrine)对雄激素依赖性前列腺癌细胞株(LNCaP)的增殖及雄激素受体(androgen re-ceptor,AR)表达的抑制作用。方法:分别用0.5、1.0、1.5、2.0、3.0g/L浓度的苦参碱作用于LNCaP细胞12、24、36h后MTT法检测细胞生长活性;台盼蓝拒染法测定细胞生长曲线;24h后流式细胞仪测定细胞周期变化;24h后Western印迹法检测细胞内AR的表达。结果:苦参碱能抑制LNCaP细胞的生长,呈剂量与时间依赖性,不同浓度苦参碱组之间与不同作用时间组之间的差异均有显著性意义(P<0.01)。苦参碱诱导LNCaP细胞出现剂量依赖性G2/M期阻滞(P<0.01);细胞内AR的表达随苦参碱剂量依赖性减少(P<0.01)。结论:苦参碱通过下调细胞内AR表达和阻滞细胞周期进展来抑制LNCaP细胞的体外生长。Objective： To study the inhibitory effect of matrine on the proliferation of the prostate cancer cell line LNCaP and the expression of the androgen receptor （AR）. Methods： LNCaP cells were treated with matrine at the concentration of 0.5, 1. 0, 1. 5, 2.0 and 3.0 g/L for 12,24 and 36 hours, the cell growth activity determined by MTT colorimetry and trypan blue staining at 36 hours, the cell cycle changes detected by flow cytometery and the expression of AR by Western blot at 24 hours. Results： Matrine suppressed the in vitro growth of the androgen-sensitive prostate cancer cell line LNCaP in a time- and dose-dependent manner, blocked the cell cycles in the G2/M phase and decreased the expression of AR in the cell line in a dose-dependent manner （P 〈 0.01 ）. Conelusion ： Matrine can significantly inhibit the in vitro growth of NCaP cells by down-regulating the expression of AR and blocking cell cycles.

关 键 词：前列腺癌 苦参碱 细胞株 LNCaP 雄激素受体 

分 类 号：R737.25[医药卫生—肿瘤] Q813.11[医药卫生—临床医学]