Mitigated calcification of glutaraldehyde-fixed bovine pericardium by tannic acid in rats  

作　　者：WANG De JIANG Hong LI Jun ZHOU Jian-ye HU Sheng-shou 

机构地区：[1]Research Center for Cardiovascular Regenerative Medicine, Ministry of Health, Cardiovascular Institute and Fuwai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100037, China

出　　处：《Chinese Medical Journal》2008年第17期1675-1679,共5页中华医学杂志（英文版）

摘　　要：Background Bioprosthetic heart valves derived from glutaraldehyde （Glut）-fixed xenografts have been widely used to replace diseased cardiac valves. However, calcification and degeneration are common following their implantation. Inflammation is closely associated with calcification of Glut-fixed xenografts via macrophage infiltration. Tannic acid （TA） possesses anti-inflammatory effects. This study was designed to investigate the anti-calcification of TA treatment on the Glut-fixed bovine pericardium （BP） in a rat subdermal model. Methods Fresh BP was divided into two groups （10 in each group） and separately subjected to different fixation procedures as follows： （1） Glut group： fixation with 0.6% Glut alone; （2） Glut/TA group： fixation with 0.6% Glut and subsequent 0.3% TA. Then the BP samples were subdermally implanted in juvenile male Sprague-Dawley rats and explanted 21 days after implantation. Each explanted BP sample was divided into three parts for calcium content analysis von Kossa＇s staining and immunohistochemical staining, and reverse transcription-polymerase chain reaction study. Results The data from quantitative calcium analysis and von Kossa＇s staining showed that Glut-fixed BP developed significantly more calcification than Glut/TA-fixed BP （（90.3＋32.5） mg/g dry weight vs （6.4_＋1.3） mg/g dry tissue, P 〈0.01）. Immunostaining demonstrated lower matrix metalloproteinase-9 and tenascin-C expression as well as macrophage infiltration into Glut/TA-fixed BP than in its Glut-fixed counterpart （P 〈0.01 for all）. Additionally, the reverse transcription-polymerase chain reaction study showed that higher levels of expression of matrix metalloproteinase-9 and tenascin-C mRNA occurred within Glut-fixed BP than within the Glut/TA-fixed ones （P 〈0.01 for all）. Conclusion TA exerts excellent anti-calcification effects on Glut-fixed BP via inhibiting macrophage infiltration and expression of matrix metalloproteinase-9 and tenascin-C.Background Bioprosthetic heart valves derived from glutaraldehyde （Glut）-fixed xenografts have been widely used to replace diseased cardiac valves. However, calcification and degeneration are common following their implantation. Inflammation is closely associated with calcification of Glut-fixed xenografts via macrophage infiltration. Tannic acid （TA） possesses anti-inflammatory effects. This study was designed to investigate the anti-calcification of TA treatment on the Glut-fixed bovine pericardium （BP） in a rat subdermal model. Methods Fresh BP was divided into two groups （10 in each group） and separately subjected to different fixation procedures as follows： （1） Glut group： fixation with 0.6% Glut alone; （2） Glut/TA group： fixation with 0.6% Glut and subsequent 0.3% TA. Then the BP samples were subdermally implanted in juvenile male Sprague-Dawley rats and explanted 21 days after implantation. Each explanted BP sample was divided into three parts for calcium content analysis von Kossa＇s staining and immunohistochemical staining, and reverse transcription-polymerase chain reaction study. Results The data from quantitative calcium analysis and von Kossa＇s staining showed that Glut-fixed BP developed significantly more calcification than Glut/TA-fixed BP （（90.3＋32.5） mg/g dry weight vs （6.4_＋1.3） mg/g dry tissue, P 〈0.01）. Immunostaining demonstrated lower matrix metalloproteinase-9 and tenascin-C expression as well as macrophage infiltration into Glut/TA-fixed BP than in its Glut-fixed counterpart （P 〈0.01 for all）. Additionally, the reverse transcription-polymerase chain reaction study showed that higher levels of expression of matrix metalloproteinase-9 and tenascin-C mRNA occurred within Glut-fixed BP than within the Glut/TA-fixed ones （P 〈0.01 for all）. Conclusion TA exerts excellent anti-calcification effects on Glut-fixed BP via inhibiting macrophage infiltration and expression of matrix metalloproteinase-9 and tenascin-C.

关 键 词：tannic acid CALCIFICATION MACROPHAGE PERICARDIUM bioprosthesis xenograft 

分 类 号：R542.5[医药卫生—心血管疾病]