青蒿琥酯对人子宫颈癌细胞株HeLa凋亡与增殖的影响  

Growth inhibition effects of artesunate on human cervical cancer cell line HeLa

作　　者：王明华[1] 郑军生[2] 骆云鹏[3] 米粲[1] 

机构地区：[1]重庆医科大学基础医学院病理学教研室,重庆400016 [2]中山大学附属第三医院妇产科,广州510630 [3]重庆医科大学基础医学院病理生理学教研室,重庆400016

出　　处：《第三军医大学学报》2008年第18期1730-1732,共3页Journal of Third Military Medical University

摘　　要：目的研究青蒿琥酯(artesunate,ART)对人子宫颈癌细胞HeLa凋亡与增殖的影响。方法通过MTT法观察ART对HeLa细胞的生长抑制作用;荧光染色观察ART作用前后细胞形态的改变;免疫组化检测HeLa细胞caspase-3表达;流式细胞术观察ART对细胞周期的影响,并检测细胞线粒体膜电位ΔΨm和细胞内活性氧(reactive oxygen species,ROS)的改变。结果ART对HeLa细胞的生长抑制作用呈时间、剂量依赖关系;荧光染色观察见凋亡小体形成;透射电镜见细胞染色质凝聚,边集呈新月形;免疫组化检测细胞质caspase-3呈阳性表达;流式细胞术检测ART可引起HeLa细胞周期S期阻滞;细胞内ROS明显升高(P<0.01),ΔΨm显著降低(P<0.05)。结论ART作用于人子宫颈癌HeLa细胞,可能通过线粒体途径诱导细胞凋亡,抑制细胞增殖。Objective To investigate the effects of artesunate on human cervical cancer cell line HeLa. Methods The inhibitory effect on cell proliferation was assessed by MTT assay. Transmission electron microscopy and fluorescent staining were applied to demonstrate the presence of apoptosis. Cell cycle, reactive oxygen species （ROS） levels and mitochondrial membrane potential （△ψm） were examined by flow cytometry, respectively. Expression of caspase-3 was detected by immunohistochemical methods. Results Growth inhibition of ART on HeLa cells was time- and dose-dependent. Apoptotic feature was observed by transmission electron microscopy and fluorescent staining. The blocking of the cell cycle was in S-phase. The expression of caspase-3 in cytoplasm was positive. The generation of ROS increased obviously （P 〈0. 01 ） after ART treated the cells for 72 h, while the levels of △ψm were decreased （P 〈 0.05）. Conclusion Artesunate inhibits the proliferation of HeLa cells, which action may be related to increasing of the cellular ROS level and decreasing of mitochon- drial member potential, resulting in cell apoptosis.

关 键 词：青蒿琥酯 HELA细胞 氧自由基 线粒体膜电位 细胞凋亡 

分 类 号：R73-36[医药卫生—肿瘤] R737.33[医药卫生—临床医学]