正畸移动牙齿牙周膜细胞ERK通路的表达变化研究  

Research on expression of pERK in human periodontal ligament cells under orthodontic mechanical force

作　　者：陈再鸿[1] 颜德权[1] 尹美女[1] 

机构地区：[1]浙江省台州医院口腔科,临海317000

出　　处：《口腔医学》2008年第8期411-413,共3页Stomatology

摘　　要：目的探讨正畸牙齿移动牙周组织改建中细胞外信号调节激酶(ERK)通路调节的分子机制。方法体外培养因阻生或正畸需要拔除的牙齿无菌条件下刮取根中1/3的牙周膜组织,采用组织块法进行原代培养,所培养的细胞经免疫细胞化学检测Vimentin,Ck(pan)。接种塑料培养板,细胞加力0、1、6、12、24、48 h后收集细胞。Western Blot检测pERK蛋白表达。结果原代人牙周膜成纤维细胞(HPDLCs)组织块培养细胞生长缓慢,传代后细胞生长旺盛,细胞形态为纺锤形或成纤维样,胞浆透明,状态良好。Vimentin染色呈阳性,Ck(pan)染色呈阴性。加力后细胞多呈长梭形,胞浆透亮均匀,排列整齐。细胞加力后pERK在1 h无明显变化,pERK表达水平随加力作用时间延长而增高,组间比较差异有统计学意义(P<0.01),12 h后pERK的蛋白表达水平下降。结论ERK在一定压力下,参与了机械力在HPDLCs的信号转导过程,其磷酸化程度随着时间点不同产生波动。Objective To investigate the correlation between orthodontic mechanical force and the expression of pERK in human periodontal ligament cells. Methods HPDLCs were tested for Vimentin and Ck （pan） by immunocytochemistry. After subjected to cyclic strain force on 1,6, 12,24 and 48 h, protein expression of pERK was determined by Western Blot. Results After treatment of the cells, the expression of pERK was higher as time went, and it was significant（ P 〈 0.01）. The expression of pERK protein declined after 12 hours. Conclusion Mechanical stress on ERK can regulate the signal of HPDLCs. As time went, phosphated ERK was changed.

关 键 词：人牙周膜成纤维细胞 细胞外信号调节的激酶 细胞加力 

分 类 号：R783.5[医药卫生—口腔医学]