鼻咽癌淋巴结转移动物模型的建立及其转移相关标签基因的筛选  

Establishment of a nude mouse model of nasopharyngeal carcinoma lymph node metastasis and screening of the metastasis-related signature genes

作　　者：冷雷[1] 刘腾飞[1] 黄仲曦[1] 谢卫兵[1] 姚开泰[1] 

机构地区：[1]南方医科大学肿瘤研究所,广东广州510515

出　　处：《南方医科大学学报》2008年第9期1519-1522,共4页Journal of Southern Medical University

基　　金：国家自然科学基金委员会-广东省人民政府联合基金(U0732006)

摘　　要：目的建立稳定的鼻咽癌(NPC)淋巴结转移模型,筛选鼻咽癌淋巴结转移的候选标志基因。方法我们用鼻咽癌5-8F-EGFP细胞株建立了稳定淋巴结转移可视化裸鼠模型,筛选获得具有淋巴结转移潜能的5-8F-LN鼻咽癌细胞。通过文献数据挖掘获得乳腺癌,头颈部鳞癌等肿瘤的转移标签基因和淋巴结转移标签基因,结合鼻咽癌5-8F和6-10B细胞株约307个差异性表达基因得到21个重叠的基因,最终分析得到3个可能促进恶性肿瘤转移的高表达基因:ADM,IRF1和CAV1。通过荧光定量PCR验证在5-8F-EGFP,6-10B-EGFP,5-8F-LN和NP69细胞中的表达情况。结果IRF1在5-8F-EGFP、6-10B-EGFP和5-8F-LN中的表达远高于在NP69中的表达,且差别有统计学意义(P=0.008、0.022、0.006);CAV1在5-8F-EGFP中的表达高于其在6-10B-EGFP中的表达(P=0.014);ADM在4种细胞中的表达无明显差别。结论IRF1可能在鼻咽癌演进过程中起着重要作用,而CAV1在5-8F-EGFP中的相对高表达可能和其高转移特性有关。Objective To establish a nude mouse model ofnasopharyngeal carcinoma （NPC） lymph node metastasis and screen the signature genes associated with the metastasis. Methods The NPC 5-8F-EGFP cells were inoculated into nude mice, from which a 5-8F-LN cell line with lymph node metastasis potential was obtained. The lymphatic metastasis-related signature genes of breast cancer and head and neck squamous cell carcinoma Were screened by data mining method. Results The NPC cell lines 5-8F and 6-10B showed 307 differentially expressed genes by microarray analysis, from which 20 overlapping genes were identified, and 3 overexpressed genes were found with probable metastasis potential, namely the ADM, 1RFI, and CAVI genes. Quantitative RT-PCR validated the data mining results in the 5-8F-EGFP, 6-10B-EGFP, NP69, and 5-8F-LN cell lines. The 3 NPC cell lines 5-8F-EGFP, 6-10B-EGFP and 5-8F-LN showed significantly higher expressions of IRF 1 than NP69 cells （P=0.008, 0.022, and 0.006, respectively. The expression level of CAVI in 5-8F-EGFP cells was significantly higher than that in 6-10B-EGFP cells （P=-0.014）, but ADM expression showed no significant difference between the 4 cell lines. Conclusions IRF1 may play an important role in the progression of NPC. The overexpression of CAVI in 5-8F-EGFP cells can be associated with the high metastatic potential of the cells.

关 键 词：鼻咽癌 转移 可视化动物模型 签名基因 荧光定量PCR 

分 类 号：R739.63[医药卫生—肿瘤]