严密型四环素调控系统调控的表达HCV-C基因双转基因小鼠模型的建立  

Establishment of a tight tetracycline-controlled HCV-C double transgenic mouse model

作　　者：帅丽芳[1] 唐博恒[1] 张若霜[2] 赵勇[2] 杨国柱[2] 陈系古[2] 

机构地区：[1]广州军区疾病预防控制中心．广东广州510517 [2]中山大学实验动物中心,广东广州510080

出　　处：《南方医科大学学报》2008年第9期1530-1533,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(103055)

摘　　要：目的制备严密型四环素调控系统调控下表达丙型肝炎病毒核心蛋白(HCV-C)基因的双转基因小鼠,为进一步阐明核心蛋白Core与HCV感染所致的疾病以及与肝细胞癌发生的关系奠定基础。方法严密型四环素调控部分(ApoE-rtTA-tTS)转基因阳性鼠和反应部分(TRE-HCV-C)转基因阳性鼠交配产生仔鼠,经PCR和Southern blot分析筛选出阳性鼠,免疫组织化学检测HCV-C蛋白双转基因小鼠肝组织内表达情况及其肝脏的病理变化。结果得到了2只携带有两种基因(tTS和HCV-C)的双转基因小鼠,成功制备了严密型四环素调控系统的HCV-C双转基因小鼠。结论结果表明,所建立的严密型四环素调控系统在动物模型中是可行的和有效的,它消除了普通四环素调控系统存在的本底泄漏表达的缺点,是HCV-C基因功能研究及与肝细胞癌的关系的机制研究的一个有用工具。Objective To develop a tight tetracycline-controlled HCV-C double transgenic mouse model. Methods By crossbreeding of ApoE-rtTA-tTS transgenic mice with TRE-HCV-C transgenic mice, the double transgenic mice were produced in the F1 generation. The presence of HCV-C and tTS gene in the FI generation was confirmed by PCR, followed by further identification and quantification of the transgene using Southem blot hybridization. The expression of HCV-C in the liver of the mouse model was detected immunohistochemically. Results and Conclusion Two transgenic mice were obtained, which contained ApoE-rtTA-tTS and TRE-HCV-C genes in the genome. Five founders contained HCV-C gene as confirmed by PCR and Southem blot hybridization. The tight tetracycline-controlled system may facilitate further study of HCV-C gene expression and gene therapy of hepatic cellular carcinoma.

关 键 词：双转基因小鼠 HCV-C基因 严密型四环素调控系统 

分 类 号：R-332[医药卫生]