黑曲霉菌株6042饲用酸性蛋白酶的性质及其应用研究  

Studies on the characteristics of feed acid proteinase by Aspergillus niger strain 6042 and its application

作　　者：费笛波[1] 钱玉英[1] 叶玲玲[1] 许尧兴[1] 冯观泉[1] 

机构地区：[1]浙江省农业科学院微生物研究所

出　　处：《浙江农业学报》1997年第6期300-304,共5页Acta Agriculturae Zhejiangensis

摘　　要：对黑曲霉变异菌株６０４２产生的酸性蛋白酶的性质及其作饲料添加剂的应用研究表明：酶作用条件基本与动物消化道生理条件相适应，最适ｐＨ为３．０～３．５，最适温度为４０～５０℃。酶液在４０℃以下稳定，５０℃以上则不稳定，６０℃处理３０分钟基本失活。固体粗酶制品的稳定性较好，室温保存半年酶活仅损失１３．４％。在最佳作用条件下，对酵母和鱼粉具有明显的降解作用，氨基酸总量分别比对照增加８９．７％和６１．８％。将５０００ｕ／ｇ的饲用酸性蛋白酶制剂以０．１％添加于乳猪及仔猪饲料中，日增重和饲料转化率分别比对照提高７．７７％和４．７１％。This study was conducted to determine some characteristics of acid proteinase produced by Aspergillus niger strain 6042 and its effect as feed additive. The conditions for enzyme action was fundamentally suited to the physiological conditions of digestion channel in animal. The optimum pH was 3.0～3.5 and the optimum reactive temperature was 40～50℃. The enzyme liquor was stable below 40℃ and unstable above 50℃. The enzyme activity was almost lost at 60℃ for 30 min. The stability of solid crude enzyme product was better and the enzyme activity was reduced by 13.4% when the solid crude enzyme powder was stored at room temperature for six months. Under the optimal conditions for enzyme action, the notable degradation function exists in decomposing yeast and fish meal. The toatl quantities of amino acid was 89.7% and 61.8% higher than that of the control respectively. While the feed acid proteinase containing 5000u/g was added to the feedstuff with the proeinase of 0.1% in sucking pig and piglet, the increase in weight each day and the rate of feed change were 7.77% and 4.71% higher than those of the control respectively. These results suggest that the acid proteinase can be applied as an ideal feed enzyme.

关 键 词：黑典霉 酸性蛋白酶 酶制剂 饲料添加剂 

分 类 号：S816.79[农业科学—饲料科学]