非小细胞肺癌微血管构筑二维表型构建方法初探  

Construction of 2-dimensional tumor microvascular architecture phenotype in non-small cell lung cancer

作　　者：刘进康[1] 王小宜[1] 熊曾[1] 周晖[1] 周建华[2] 傅春燕[2] 李波[2] 

机构地区：[1]中南大学湘雅医院放射科,长沙410008 [2]中南大学湘雅医院病理科,长沙410008

出　　处：《中南大学学报（医学版）》2008年第8期712-717,共6页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(07JJ5010)~~

摘　　要：目的:探讨肿瘤微血管构筑二维表型(2D-TMAP)表达技术平台构建方法。方法:完整地采集30例非小细胞肺癌肺内结节标本,按其活体解剖方位选取与多层螺旋CT灌注成像最大层面相当的病理层面,采用免疫组织化学、特殊染色(GMS)、电镜观察等技术构建该病理层面2D-TMAP,检测指标包括微血管形态、微血管基底膜完整性、微血管密度、微血管不同表型与微淋巴管、微血管成熟度与管腔化程度以及微血管免疫遗传学特性。结果:成功构建2D-TMAP表达技术平台,2D-TMAP在非小细胞肺癌组织中呈异质性表达,非小细胞肺癌微脉管超微结构具有一定的特征。结论:2D-TMAP能观察肿瘤生长微环境的整体状态,是探讨肺内结节性病变血管新生和血流模式差异的关键技术。Objective To construct cular architecture phenotype （ 2 D-TAMP ） lung cancer （NSCLC） were collected after a technological platform of 2-dimensional tumor microvasexpression. Methods Thirty samples of non-small cell surgery. The corresponding sections of tumor tissue specimens to the slice of CT perfusion imaging were selected. Immunohistochemical staining, Gomori methenamine silver stain, and electron microscope observation were performed to build a technological platform of 2 D-TMAP expression by dectecting the morphology and the integrity of basement membrane of microvasculature, microvascular density, various microvascular subtype, the degree of the maturity and lumenization of microvasculature, and the characteristics of immunogenetics of microvasculature. Results The technological platform of 2D-TMAP expression was constructed successfully. There was heterogeneity in 2 D-TMAP expression of non-small cell lung cancer. The microvascular of NSCLC had certain characteristics. Conclusion 2D-TMAP is a key technology that can be used to observe the overall state of micro-environment in tumor growth.

关 键 词：非小细胞肺癌 微血管构筑表型 异质性 

分 类 号：R734.2[医药卫生—肿瘤]