Mg对单纯疱疹病毒性脑炎小鼠水孔蛋白-4表达的影响及血脑屏障通透性的研究  

Magnesium protects BBB via reducing the expression of AQP4 in herpes simplex encephalitis mice

作　　者：周瑜[1] 郭远瑾[1] 刘璇[1] 梅元武[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,湖北武汉430022

出　　处：《中风与神经疾病杂志》2008年第4期423-425,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究镁剂对单纯疱疹病毒性脑炎(Herpes simplex encephalitis,HSE)后水孔蛋白-4(aquaporin-4,AQP4)表达、血脑屏障(BBB)通透性及神经功能的影响。方法小鼠脑内接种HSV-1病毒,治疗组静脉注射硫酸镁,对照组仅注射等量生理盐水。采用免疫组化技术对感染侧脑组织AQP4蛋白进行检测。通过检测渗出到脑血管外的伊文斯蓝(Evans Blue,EB)的含量来定量观察BBB的通透性。HSV-1病毒感染后5d对小鼠进行神经功能评分。结果造模后第3天,脑组织AQP4的表达增加,第5天时更加明显(P<0.01),与血脑屏障通透性增高相一致,给与硫酸镁治疗后,血脑屏障通透性及AQP4的表达均明显降低(P<0.01),伴随着神经功能的改善。结论镁剂能抑制AQP4蛋白的表达,保护BBB,改善脑炎后神经功能。Objective To investigate the effects of magnesium on the AQP4 protein expression and the roles of AQP4 protein in brain edema formation in Herpes simplex encephalitis （HSE）. Methods Induction of encephalitis in BALB/c mice by intracerebral inoculation with herpes simplex virus type 1 （ HSV-1 ）. The treated group was administered magnesium sulphate（350 μmol/kg） via the femoral vein,while injected equal volume saline vehicle in control group. Immunohistochemistry method was adopted to analyse the expression of AQP4 protein in the infected brain tissue. The blood-brain barrier（BBB） permeability was evaluated quantitatively by measuring Evans blue dye extravasations. Neurological Score was quantified using an established neurological scale on 5d after HSE. Results After HSV-1 infection,the AQP4 protein performed significantly enhanced level in the control group on 3d, especially on 5d after HSE（P 〈 0.01 ）. The results were concordant with the increased BBB permeability. The mice treated with magnesium sulphate significantly attenuated AQP4 expression compared with control group（P 〈 0.01 ）. Magnesium sulphate also protected the BBB permeability and improved clinical outcome. Conclusion Magnesium sulphate protected BBB permeability after HSE by downregulating the expression of AQP4 protein.

关 键 词：单纯疱疹病毒性脑炎 血脑屏障 镁 水孔蛋白-4 

分 类 号：R743.3[医药卫生—神经病学与精神病学]