X线对脑胶质瘤细胞系增殖的影响  

EFFECT OF X-ray RADIATION ON PROLIFERATION OF HUMAN GLIOMA CELLS

作　　者：刘承勇[1] 杨开军[1] 任文德[1] 漆松涛[1] 许志新[1] 武华坪[1] 

机构地区：[1]第一军医大学南方医院

出　　处：《解放军医学杂志》1997年第6期419-421,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：为探讨不同Ｘ线处理对脑胶质瘤细胞增殖的影响，选用人脑星形细胞瘤ＳＷＯ－３８系，采用ＭＴＴ法观察Ｘ线对胶质瘤细胞生长的影响，ＡＢＣ法检测增殖细胞核抗原（ＰＣＮＡ）基因表达的变化。结果显示：①不同剂量（５，１０，１５，２０，２５Ｇｙ）Ｘ线对胶质瘤细胞生长具有明显的抑制作用，生长曲线低平，照射后４８ｈ的抑制率分别为３３％、４７％、５１％、５８％和６６％；②不同剂量率（８０，１６０，２４０，３２０，４００ｃＧｙ／ｍｉｎ）Ｘ线照射后４８ｈ的抑制率分别为５８％、５４％、４７％、２５％和３０％；③超分割照射后４８ｈ的抑制率为６２％，而单次照射组为５０％；④不同剂量（５，１０，１５，２０，２５Ｇｙ）Ｘ线照射后ＰＣＮＡ－标记率（ＬＩ）分别为９１％，８７％，８１％，７８％，６９％，ＰＣＮＡ－ＬＩ与存活细胞ＯＤ值呈显著性正相关关系（Ｐ＜０．０５）。说明①ＳＷＯ细胞生长曲线、抑制率与Ｘ线剂量、剂量率和照射方式之间密切相关；②ＰＣＮＡ－ＬＩ与存活细胞ＯＤ值呈正相关关系，ＰＣＮＡ是胶质瘤细胞增殖的标志之一。To investigate the effect of radiation by Xray on human glioma cells, modified MTT assay was adopted to observe the survival curves and inhibition rates of SWO cell lines after Xray radiation with the different modes. The results showed that: (1) There were significant inhibitive effects after different dose(5,10,15,25 Gy) Xray radiation on SWO cell lines. The inhibitive rates were 33%, 47%, 51%, 58% and 66% respectively after 48 hours of radiation. (2) The inhibitive rates were 58%, 54%, 47%, 25% and 30% respectively after 48 hours of the different doserate(80,160, 240, 320, 400cGy/min) radiation. (3) The inhibitive rates were 62% after hyperfractionational radiation, 50% for singlefractionation radiation. (4)PCNALI were respectively 91%, 87%, 78%, 69% after different dose(5,10, 15,20,25Gy)Xray radiation. PCNALI had significantly positive correlation with the OD value of survival cells (P<005). It was suggested that:(1)The survival curves and inhibition rates were closely related to the dose, doserate and radiation mode. (2)PCNALI was an important index of tumor cell susceptibility to radiation, and a symbol of S/G1 phrase in mitosis.

关 键 词：放射疗法 神经胶质瘤 X线 脑肿瘤 

分 类 号：R739.410.5[医药卫生—肿瘤]