DSPP基因启动子不同片段启动活性的对比分析  

The promoter activity of different segments of mouse dentin sialophosphoprotein promoter.

作　　者：郭婷[1] 曹罡[1] 余擎[2] 肖明振[2] 赵守亮[2] 吉兰[2] 

机构地区：[1]南京军区总医院口腔科,江苏南京210002 [2]第四军医大学口腔医学院

出　　处：《临床口腔医学杂志》2008年第8期458-460,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助(30271418);南京军区总医院资助项目(2006052)

摘　　要：目的:对比分析牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)基因启动子不同片段的启动活性。方法:选择小鼠成牙本质细胞样细胞MDPC-23为实验细胞,利用含DSPP基因启动子不同片段的真核表达载体,采用报告基因方法观察DSPP基因启动子不同片段的启动活性。结果:在MDPC-23细胞中,DSPP基因启动子不同片段的真核表达载体均不同程度地表现了启动活性,其启动活性有差异(P<0.01)。结论:DSPP基因启动子不同片段的启动活性有差异,其活性变化反映位于-95bp～54bp、-410bp～-195bp、-1243bp～-791bp、-1447bp～-1243bp、-3519bp～-2475bp区存在潜在的增强子;-195bp～-95bp、-670bp～-410bp、-2475bp～-1447bp区存在潜在的抑制子。进一步明确了DSPP基因启动子的结构,为今后研究DSPP基因的特异性表达奠定基础。Objective： To study the difference of promoter activity of different segments of mouse dentin sialophos- phoprotein （DSPP） promoter. Method： After transfected report vectors of different segments of mouse DSPP promoter into MDPC-23 cells using the LipofectamineTM2000, the cells were measured for luciferase activity using the dual luciferase reporter assay system. The results were analysised by software of SPSS 10.0 for Windows. Result：In MDPC-23 cells,report vectors of different segments of mouse DSPP promoter show different promoter activity. （P〈0.01）. Conclusion： the difference of promoter activity of different segments of DSPP promoter is significant. Five potential enhancer were identified in the re- gions between nt -95 bp and 54 bp,nt -410 bp and -195 bp,nt -1243 bp and -791 bp,nt -1447 bp and -1243 bp,nt -3519 bp and -2475 bp; three potential suppresser were identified in the regions between nt -195 bp and -95 bp,nt -670 bp and - 410 bp,nt -2475 bp and -1447 bp. These results have significance for future studies of DSPP tooth-specific regulation.

关 键 词：牙本质涎磷蛋白 瞬时转染 报告基因 

分 类 号：Q786[生物学—分子生物学]