磁性阿霉素聚氰基丙烯酸正丁酯纳米粒的合成及其对MG-63人骨肉瘤细胞的效应  

Synthesis of Adriamycin Polybutylcyanoacrylate Magnetic Nanoparticles and Its Biological Effects on MG-63 Human Osteosarcoma Cells

作　　者：王栋峰[1] 蔡林[1] 胡治平[1] 胡昊[1] 金伟[1] 王建平[1] 

机构地区：[1]武汉大学中南医院骨外科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2008年第5期610-616,共7页Medical Journal of Wuhan University

基　　金：湖北省科技攻关计划基金资助课题(编号:2004AA304B08)

摘　　要：目的:利用FeCl2.4H2O、FeCl3.6H2O、α-氰基丙烯酸正丁酯、盐酸阿霉素等试剂合成磁性阿霉素聚氰基丙烯酸正丁酯纳米粒的稳定胶体液,并研究其对MG-63型人骨肉瘤细胞的作用。方法:①用FeCl2.4H2O、FeCl3.6H2O、盐酸阿霉素、α-氰基丙烯酸正丁酯等试剂合成磁性阿霉素聚氰基丙烯酸正丁酯纳米粒。②在透射电镜下观察纳米粒的物理形态;用振动样品磁强计检测纳米粒的饱和磁化强度;用紫外分光光度计测定药物的包封率、载药量。③用扫描电镜观察、MTT法和流式细胞仪测定法检测其对MG-63型人骨肉瘤细胞的作用。结果:利用FeCl2.4H2O、FeCl3.6H2O、盐酸阿霉素、α-氰基丙烯酸正丁酯等试剂成功合成了一种稳定的棕色胶体溶液;透射电镜下观察,形态近似于球形,分散度好,平均直径184.60 nm;振动样品磁强计检测胶体中纳米粒的饱和磁化强度为0.558 emu/g,紫外分光光度计等仪器测定药物的包封率为90.73%,载药量为10.68%;通过扫描电镜、MTT法和流式细胞仪测定法检测,表明该胶体溶液较ADM对MG-63人骨肉瘤细胞有更强的抑制作用。结论:该胶体溶液性质稳定,药物颗粒不超过300 nm,理论上可以在动物血管中随循环流动而不会沉淀;该纳米粒的饱和磁化强度为0.558 emu/g,微粒可被4 250 Gs的磁场所吸引,以达到磁靶向的效应;药物的包封率为90.73%,载药量为10.68%,表明药物合成的效率较好;且该胶体溶液较ADM对MG-63人骨肉瘤细胞有更强的抑制作用,为下一步动物实验提供了基础。Objective： To synthesize a kind of stable colloid solution of adriamycin polybutylcyanoacrylate magnetic nanoparticles and to study its effects on MG-63 type human osteosarcoma cells. Methods. ①A kind of stable colloid solution of adriamycin polybutylcyanoacrylate magnetic nanoparticles was synthesized with FeCl2·4H20, FeCl3·6H2O, α-polybutylcyanoacrylate, adriamycin hydrochloride,etc.②Particles were observed under transmission electronic microscope. The saturation magnetization was detected with vibration essay Gauss meter. The envelopment rate and drug carried rate was determined with violet spectrophotometer. ③SEM, MTT and FCM methods were adopted to study the effect on MG-63 human osteosarcoma cells. Results： A kind of brown stable colloid solution of adriamycin polybutylcyanoacrylate magnetic nanoparticles was synthesized. Under TEM, the particles were round in shape, their dispersity was very well, and the average diameter was 184.60 nm. Their saturation magnetization was 0. 558 emu/g, the envelopment rate was 90.73%, drug carried rate was 10.68% and the colloid solution had done inhibition effects （compared with adriamycin） on MG 63 human osteosarcoma cells. Conelusion： The colloid solution is stable, and the diameter of the drug particle is less than 300 nm, so it can flow in the blood-vessel without block. The particles＇ saturation magnetization, envelopment rate and drug carried rate was suitable for the target chemo-therapy. The colloid solution had a superior inhibition effect on MG-63 human osteosarcoma cells than ADM, therefore, it offers a reliable base of the animal experiment in the future.

关 键 词：阿霉素 磁性纳米粒 合成 MG-63型人骨肉瘤细胞 

分 类 号：R738.1[医药卫生—肿瘤] R730.53[医药卫生—临床医学]