喉癌细胞株 HEp-2 性激素代谢的研究  

STUDY ON METABOLISM OF SEX HORMONES IN LARYNGEAL CARCINOMA CELL LINE HEp2

作　　者：于雪艳[1,2] 石梅[1,2] 胡晓燕[1,2] 王明运[1,2] 王廷础[1,2] 

机构地区：[1]山东医科大学生物化学教研室 [2]卫生部耳鼻喉科学重点实验室

出　　处：《山东医科大学学报》1997年第4期274-277,共4页Acta Academiae Medicinae Shandong

摘　　要：人喉癌细胞株ＨＥｐ－２经无性激素培养基（活性碳处理小牛血清）培养后，收集细胞制备匀浆，用３Ｈ标记配体测定其雄激素受体、雌激素受体，同时测定５α－还原酶（５α－Ｒ）和１７β－羟类固醇脱氢酶（１７β－ＨＳＤ）活性。结果显示，ＨＥｐ－２细胞株含雄激素受体，受体水平为１３．７３ｆｍｏｌ·Ｌ－１·ｍｇ－１Ｐｒ，未测到雌激素受体；５α－Ｒ催化睾酮还原为二氢睾酮的转换率为５０％，１７β－ＨＳＤ活性极低。提示ＨＥｐ－２喉癌细胞株仍保留表达雄激素受体的能力，而且５α－Ｒ的活性较高。HEp2 cells were cultured in MEM supplemented with 5% fetal calf serum that had been treated with dextrancoated charcoal(DCC).The havested cells were homogenized and the supernatant cytosol fraction was collected after centrifugation at 40000g at 0￣4℃ for 1 hour.Specific androgen,estrogen receptors and the activity of 5αreductase (5αR)and 17βhydroxysteroid dehydrogenase(17βHSD)were determined in HEp2 cell line by 3H label substrates respectively.The results showed that HEp2 cell line contained detectable contents specific androgen receptors(13.73 fmol·L-1·mg-1 cytosol protein)and no estrogen receptors.5αreductase permits the conversion of a less active androgen,testosterone,into a higer one,dihydrotestosterone,the conversion rate was 50%,only a few 17βHSD were determined.These observations suggested that HEp2 cell line still contain androgen receptors and has higher activity of 5αR.

关 键 词：喉肿瘤 性激素 代谢 HEP-2 细胞株 

分 类 号：R739.650.2[医药卫生—肿瘤]