纤维蛋白对大鼠脑血管内皮细胞白细胞介素6表达的影响  

Effect of fibrin on expression of interleukin-6 in rat brain vascular endothelial cells

作　　者：薛峥[1] 张苏明[1] 

机构地区：[1]华中科技大学附属同济医院神经内科,湖北武汉430030

出　　处：《中国病理生理杂志》2008年第9期1746-1750,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30700244)

摘　　要：目的:观察纤维蛋白对离体培养的大鼠脑血管内皮细胞白细胞介素6(IL-6)转录及蛋白水平表达的影响。方法:大鼠脑血管内皮细胞分离后培养,加入不同浓度的纤维蛋白,通过real-time PCR检测脑血管内皮细胞中IL-6转录水平,应用酶联免疫方法(ELISA)定量检测培养基和细胞裂解液中的IL-6水平。结果:加入不同浓度(0.03 g/L、0.1 g/L、0.3 g/L和1.0 g/L)的纤维蛋白24 h后,1.0 g/L纤维蛋白组的培养基中IL-6水平显著增高(P<0.01);然后加入1.0 g/L纤维蛋白2、4、8、24 h,培养基中的IL-6水平均显著升高(P<0.05);而加入不同浓度的胶原蛋白不引起IL-6水平的明显变化;real-time PCR结果显示,脑血管内皮细胞IL-6 mRNA的上调呈现出剂量和时间依赖性增加。结论:纤维蛋白是血管内皮细胞活化剂,可以上调大鼠脑血管内皮细胞中IL-6的表达,在蛋白和mRNA均呈现出剂量和时间依赖性增加。AIM： To investigate the kinetics of interleukin -6 （IL- 6） protein and mRNA expressions in the coculture of rat brain vascular endothelial cells（VECs） with fibrin. METHODS： The IL -6 concentration were measured by rat IL - 6 ELISA kit and the IL - 6 mRNA was measured by real - time PCR after development an in vitro model of in situ fibrin polymerization on rat brain vascular endothelial cells. RESULTS ： Fibrin induced IL - 6 expression. The IL - 6 antigen concentration increased significantly in 1.0 g/L media group compared to the low dose fibrin group or control media group （P 〈 0. 01 ）. Fibrin -induced IL -6 expression in rat brain VECs as early as 2 h post -fibrin stimulation, increased significantly at 8 h, keeping at high level till 24 h （P 〈 0. 05）. Collagen did not induce IL - 6 expression at 24 h. Real - time PCR analysis showed that 1L - 6 antigen increase in fibrin treated rat brain VECs was due to fibrin induced elevation of steady state mRNA expression. CONCLUSION ： Fibrin is a potent vascular endothelial cell activator. It induces IL -6 expression in time and dose dependent fashions.

关 键 词：纤维蛋白 脑 血管内皮细胞 白细胞介素6 大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学]