伴侣因子60在实验性急性胰腺炎肝胰组织中的表达及意义  

Expression and potential role of chaperonin 60 in experimental acute pancreatitis

作　　者：李琨[1] 李永渝[1] 李学晋[1] 李艳娜[1] 陆新元[1] 陈昌杰[1] 

机构地区：[1]同济大学医学院病理生理教研室消化系疾病研究所,上海200092

出　　处：《中国病理生理杂志》2008年第9期1804-1810,共7页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30770978)

摘　　要：目的:探讨不同严重程度的实验性急性胰腺炎(AP)时动物肝脏和胰腺组织中伴侣因子60(Cpn60)的表达特点及可能的意义。方法:用雨蛙肽腹腔注射复制急性轻型胰腺炎(MAP)小鼠模型;用去氧胆酸钠逆胰胆管注射复制急性重型胰腺炎(SAP)大鼠模型;分别设各自正常对照组(control)和假手术对照组(sham组)。造模后1、5、10 h分批处死动物,取肝、胰组织。病理切片观测胰腺组织病变情况;免疫共沉淀(IP)及Western blotting技术确定肝、胰组织Cpn60蛋白条带位置及表达的改变。结果:MAP及SAP组的胰腺组织分别出现水肿性和出血坏死性改变。MAP组及其sham组1 h Cpn60表达量明显低于正常小鼠,而5 h的表达量显著升高。SAP组和相应的sham组1 h Cpn60表达量明显增高,随后降低,从1 h到10 h SAP组的下降幅度明显大于相应sham组。结果还发现,小鼠、大鼠胰腺和肝脏组织Cpn60蛋白有两条带,在各组及其不同时段,该蛋白条带的变化各有不同。结论:大鼠、小鼠胰腺和肝脏组织中Cpn60蛋白表达呈双带,且在AP时变化不一,提示AP时不仅有Cpn60蛋白量的表达异常,还可能存在质的异常,这些异常与AP发生、发展的关系有待研究。AIM： To explore the expression and potential impacts of ehaperonin 60 （ Cpn60 ） in the hepatic and pancreatic tissues from animals endured experimental acute panereatitis （AP） with various severities. METHODS： Induction of mild acute pancreatitis （MAP） in mice was made by intraperitoneal injection of eaerulein, and sodium deoxycholate was used ＇by injection through panereato - biliary duet backward to induce severe acute panereatitis （SAP） in rats. The liver and pancreas from sacrificed animals at 1 h, 5 h and 10 h time points post - induction of AP were harvested for pathological examination and observing the dynamic change of Cpn60 expression with techniques of immunoprecipitation （IP） and Western blotting. RESULTS： In the MAP and SAP models, pancreatic tissues showed swollen or hemorrhagic necrotic changes, respectively. The characteristic differences of Cpn60 expression were also observed. The Cpn60 protein was expressed as two distinctive bands in pancreatic and hepatic tissues, and relative densities of the two bands varied differently at these time points in both AP models. CONCLUSION ： The results suggest that not only the quantitative, but also, probably, qualitative abnormalities of Cpn60 expression in AP exist. These abnormalities may play important roles in the pathogenesis and development of acute pancreatitis.

关 键 词：胰腺炎 伴侣因子60 胰腺 肝 

分 类 号：R363.2[医药卫生—病理学]