口蹄疫病毒基因组的遗传变异剖析  

Dissection on Genetic Diversiy of Foot-and-Mouth Disease Virus Genomes

作　　者：陈豪泰[1] 张杰[1] 孙德惠[1] 马丽娜[1] 刘湘涛[1] 刘永生[1] 

机构地区：[1]中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/农业部畜禽病毒学重点实验訇农业部草食动物疫病重点开放实验室,兰州730046

出　　处：《中国农业科学》2008年第9期2826-2834,共9页Scientia Agricultura Sinica

基　　金：甘肃省科技重大专项计划项目(0801NKDA034);兰州市科技计划项目(07-2-12、和2008-1-167);甘肃省自然基金(0803RJZA050)

摘　　要：【目的】明确口蹄疫病毒基因组的结构特征及其变异与结构、功能的关系以及系统发生关系。【方法】利用DNAstar和Clustalx程序进行184个口蹄疫病毒基因组序列的同源性分析、多重排比。【结果】口蹄疫病毒基因组ORF大小有所差异,范围为6963～7120 nt,编码2320～2339 aa的多聚蛋白。核苷酸和氨基酸序列的同源性,7个不同血清型间>77.6%和>78.3%,本研究发现了可能和生物学功能相关的新的保守和变异区域。【结论】口蹄疫病毒RNA的变异类型丰富和多样性程度较高,自然界存在的毒株可能大于血清学和测序发现的FMDV的毒株数目。[Objective] The purpose of this study is to clarify the structural features of foot-and-mouth disease virus （FMDV） genomes, relationships of the sequence variations with its structure-function, and molecular phylogeny among FMDV. [Method] The identity analysis and multiple alignment of 184 FMDV genome sequences were undertaken, respectively, by using the DNAstar and Clustalx packages. [Result] FMDV genome sequences showed the entire ORFs size range from 6 963 to 7 120 nt, which could encode PrPs with 2 320 to 2 339 aa. The homology of these nucleotide and amino acid sequences were greater than or equal to 77.6% and greater than or equal to 78.3% among seven distinct serotypes, respectively. The data reveal novel highly conserved genomic regions, indicating variability as well as novel viral genomic motifs with likely biological relevance. [Conclusion] These results suggest that more FMDV genome diversity may exist in nature than is currently indicated by serology or sequence analysis.

关 键 词：口蹄疫 遗传变异 病毒基因组 结构特征 

分 类 号：S852.65[农业科学—基础兽医学]