当归芍药散神经保护作用的实验研究  

Study on the neuroprotection of Danggui Shaoyao San(DSS)

作　　者：田建英[1] 马锋[1] 马伟[1] 马丽君[1] 

机构地区：[1]宁夏医学院,宁夏银川750004

出　　处：《山东医药》2008年第23期1-3,共3页Shandong Medical Journal

基　　金：国家教育部优秀青年教师资助项目(教人司2002350号);国家中药管理局资助项目(04-05zp62)

摘　　要：目的观察当归芍药散（DSS）的神经保护作用，为阿尔茨海默病（AD）防治提供理论依据。方法按DSS经典方制备DSS水煎剂后以5％、10％、20％三种体积分数分别灌服家兔1d和10d（观察1组～观察6组），对照组灌服等量生理盐水。末次灌胃给药1h后处死家兔，由枕骨大孔处抽取脑脊液（DSS脑脊液和空白脑脊液）。分别采用上述各组脑脊液及Aβ1-40处理嗜铬细胞瘤细胞株PC12细胞，MTT比色法观察细胞活性变化，免疫组化法观察α7烟碱型乙酰胆碱能受体（α7nAchR）阳性表达情况。结果β1-40处理者PC12细胞活性和α7nAchR阳性细胞数明显小于空白脑脊液处理者（P〈0．01）；灌服20％DSS脑脊液处理者PC12细胞活性及α7nAchR阳性细胞数明显高于Aβ1-40处理者。结论DSS可能通过抑制β-淀粉样多肽的神经毒性作用而改善AD患者的学习记忆功能，机制为调节细胞α7nAChR表达。Objective To study the neuroprotective mechanism of Danggui Shaoyao San （DSS） on Alzheimer disease （AD）. Methods 42 white rabbits were randomly derided into seven groups,observed group 1 to 6 were filled the stomach with DSS liquid of 5% ,10% ,20% for 1 or 10 days respectively,the control group was treated with equal volume 0.9% saline water. Aβ1-40 and cerebrospinal fluid （CSF） of the seven groups were used to treat the PC12 cell. The protein ex- pression of nAchRα7 and cell viability were detected by immunochemistry and MTT assay. Results The cell viability of PC12 cell and expression of nAchRα7 treated with Aβ1-40 were lower than those treated with CSF from control group（ P 〈 0.01 ）, and higher than those treated with CSF from observed group treated with DSS liquid of 20% （P 〈 0.01 ）. Conclusions DSS can improve the learning and memory ability of cases with AD, the probable mechanism maybe suppress the neurotoxine of Aβ and regulate the expression of nAchRα7.

关 键 词：α7烟碱型乙酰胆碱能受体 当归芍药散 

分 类 号：R749.1[医药卫生—神经病学与精神病学]