小鼠骨髓源性树突状细胞的体外扩增、鉴定及生物学特性研究  

Study of generation,identification and cytobiological characteristics of dendritic cells from mouse bone marrow

作　　者：苏伟[1] 孙宗全[1] 董念国[1] 杜心灵[1] 蒋雄刚[1] 陈家军[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《山东医药》2008年第23期12-14,共3页Shandong Medical Journal

基　　金：国家自然基金资助项目(30471715)

摘　　要：目的为树突状细胞（DC）的研究和应用奠定基础。方法以重组小鼠粒细胞-巨噬细胞集落刺激因子（GM-CSF）、白细胞介素（IL-4）和脂多糖（LPS）体外诱导小鼠骨髓细胞分化为DC，倒置显微镜动态观察细胞形态学变化，流式细胞术分析细胞表面分子，混合淋巴细胞反应检测其刺激T细胞增殖能力。结果经体外诱导培养第2—8天可见大量细胞集落形成；获得的DC具有典型树突状形态，同时DC可显著刺激同种异体混合淋巴细胞增殖。结论体外诱导培养可获得小鼠骨髓来源DC，可广泛应用于临床及实验研究。Objective To investigate the cytobiological characteristics of dendritic cell （DC） for its experimental study and clinical application. Methods Mouse bone marrow cells were induced to form DCs by recombinant mouse-granulocyte and macrophage colony-stimulus factor （GM-CSF）, interleukin-4 （IL-1） and lipopolysaccharide （LPS） in vitro. The morphological changes were observed with light inverted microscope. Cell surface molecules were identified by flow cytometry. The biological function was studied with mixed lymphocyte reaction （MLR）. Results The cultured cells displayed typical morphological characteristics of DCs,and could stimulate the proliferation of allogenic mixed lymphocyte. Conclusions In vitro induction and culture can obtain DCs, and it can be widely used in the experimental and clinical study.

关 键 词：树突状细胞 细胞培养 流式细胞仪 

分 类 号：R730.3[医药卫生—肿瘤]