食管鳞癌组织中PTEN和血管内皮生长因子的表达及相关性研究  

Correlation and expression of PTEN and vascular endothelial growth factor in human esophageal carcinoma

作　　者：王永连[1] 陶义鹏[1] 王毅[1] 王忠民[1] 李汉臣[1] 赵国昌[1] 杨承汉[2] 

机构地区：[1]新乡医学院第一附属医院胸外科,河南省卫辉市453100 [2]新乡医学院第一附属医院消化科,河南省卫辉市453100

出　　处：《中国综合临床》2008年第10期983-985,共3页Clinical Medicine of China

基　　金：河南省科技厅立项课题（524410055）

摘　　要：目的通过对PrrEN和血管内皮生长因子（VEGF）在食管癌中表达的研究，结合临床病理特征，探讨PTEN和VEGF的临床意义及相关性。方法应用免疫组织化学S-P法检测80例食管癌组织及20例正常食管黏膜中PTEN和VEGF的表达。结果食管癌组织中PTEN蛋白表达率为38．75％（31／80），显著低于正常食管黏膜的表达（100％，20／20）（P〈0．01），PTEN蛋白低表达与组织分化程度密切相关（r=0．57，P〈0．05），与淋巴结转移密切相关（r=0．61，P〈0．01）。VEGF在食管癌中的表达率为70．13％（57／80），显著高于正常食管黏膜的表达（15．0％，3／20）（P〈0．01），VEGF的表达与癌组织浸润深度密切相关（r=0．49，P〈0．05），与淋巴结转移也密切相关（r=0．55，P〈0．05），与组织分化程度相关（r=0．48，P〈0．05）。PrrEN在食管癌中的表达与VEGF呈负相关（r=-0．327，P〈0．01）。结论联合检测PTEN、VEGF有助于提高食管癌侵袭转移能力的评估，对食管癌的预后判断具有重要的临床意义。Objective To investigate the expression of PTEN and vascular endothelial growth factor （VEGF） in esophageal carcinoma and the relationship between their expression. Methods The expression of PTEN and VEGF were detected using immunohistochemical S-P method, Results Among 80 cases of esophageal carcinoma, 31 showed positive staining of PTEN （ 38.75 % ） , while all 20 cases of normal mucosa showed positive staining of PTEN. The expression level of PTEN in highly differentiated squamous carcinoma was higher than that in low differentiated squamous carcinoma, Also, the expression of PTEN was significantly correlated with lymph node metastasis （ r = 0.61,P 〈 0.01 ） and differentiation（ r = 0.57 , P 〈 0.05 ）. In 80 cases of esophageal carcinoma,57 （ 70.13 % ） were of positive staining of VEGF, while in 20 of normal esophageal mucosa, only 3 showed positive staining of VEGF. The expression of VEGF was markedly correlated with infiltrative deepness （ r = 0.49, P 〈 0.05 ） and lymph node metastasis （ r = 0.55 , P 〈 0.05 ） and differentiation （ r = 0.48, P 〈 0.05 ）. Conclusion Combined detection of PTEN and VEGF maybe helpful to evaluate prognosis and infiltrative capability of esophageal carcinoma,with significant importance to the arediction of the prognosis of esophageal carcinoam.

关 键 词：食管癌 血管内皮生长因子 PENT基因 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]