头部药物注射疗法对大鼠缺氧缺血性脑损伤后神经细胞和神经胶质细胞的影响  

Effects of epicranium medication injection of VitB_1 and VitB_(12) on neurons and astrocytes of hypoxic-ischemic brain damage rats

作　　者：刘素芳[1] 万国兰[2] 朱登纳[2] 王军[2] 闫丙川[2] 钟延丰[3] 

机构地区：[1]郑州大学基础医学院生理学教研室,郑州450001 [2]郑州大学第三附属医院脑瘫康复治疗中心,郑州450052 [3]北京大学医学部病理学系,北京100083

出　　处：《郑州大学学报（医学版）》2008年第5期909-911,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关基金资助项目0324410031

摘　　要：目的:探讨维生素B1和维生素B12位点注射疗法对缺氧缺血性脑损伤(HIBD)后大鼠神经细胞和神经胶质细胞的影响。方法:选取27只Wistar大鼠制成HIBD动物模型,20d后随机分为A、B、C3组(n=9),同时设D组(不构建模型)为正常对照组(n=5)。A组固定于额顶叶处注射维生素B1及维生素B12,1次/d,共25d;B组功能训练(鲍巴斯法);C组不予处理。观察各组动物的一般情况,50d后处死动物取脑进行电镜观察。结果:D组一般情况正常,A、B、C组大鼠体质量增长迟缓,皮毛干燥无光泽,出现行为能力障碍,如过度兴奋、惊吓、易激惹等。电镜下可见A组神经细胞结构较好,星形细胞足突空泡变性与膜破坏及小血管变化较轻。B组神经细胞轻度变性,星形细胞足突空泡变性与膜破坏及小血管变化较明显。C组神经细胞重度变性,呈现重度暗细胞表现,星形细胞足突空泡变性与膜破坏及小血管变化明显。结论:HIBD模型可产生类似脑瘫的病理改变,维生素B1和维生素B12位点注射有改善神经细胞和星形胶质细胞的作用,具有修复功能。Aim ： To study the effects of epicranium medication injection of VitB1 and VitB12 on neurons and astrocytes of hypoxic-lschemic brain damage rats. Methods ： A total of 27 Wistar rats were chosen to establish animal models of HIBD. Twenty days later, the rats were divided into three groups randomly（ group A, B,and C,n = 9）. Group D was used as normal control group（n =5）. Group A was treated with VitB1 and VitB12 by injecting over frontal lobe and apical lobe once a day for 25 days. Group B was treated with function training（ Bobath methods）. Group C was not given any treatment. After 50 days, all the animals were killed and their brains were taken to observe the pathological changes under the electron mi- croscope. Results：Under electron microscope： neurons and astrocytes in group A had less denaturalization. Group B and C had more vacuole appearance, even dark cells. But group C had obvious abnormal changes. Conclusion： HIBD model can form pathological process similar to cerebral palsy and eplcranium medication injection of VitB1 and VitB12 relieve the injury of nerve cells and has better repairing effects.

关 键 词：缺氧缺血性脑损伤 额叶 顶叶 神经细胞 大鼠 

分 类 号：R742.3[医药卫生—神经病学与精神病学]