碱性成纤维细胞生长因子基因转染对虹膜色素上皮细胞增殖及细胞周期的影响  

Measure of mitogenesia and S-cycle in bFGF-mediated transgenic iris pigment epithelial cells

作　　者：杨晓慧[1] 孙葆忱[2] 

机构地区：[1]郑州大学第一附属医院眼科,郑州450052 [2]首都医科大学附属北京同仁医院北京市眼科研究所,北京100005

出　　处：《郑州大学学报（医学版）》2008年第5期978-980,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:了解重组腺相关病毒(rAAV)载体介导碱性成纤维细胞生长因子(bFGF)基因转染对体外培养的虹膜色素上皮细胞(IPE)增殖及细胞周期的影响。方法:在体外培养的IPE细胞中加入转染rAAV-bFGF基因细胞和未转染细胞的培养液上清,继续培养4h或1d后分别应用MTT法和流式细胞仪检测IPE细胞的生长状况。结果:MTT法结果显示,加入rAAV-bFGF基因转染组培养液上清的IPE细胞D值为0.338±0.047,对照组为0.277±0.064,2组相比,t=3.745,P<0.05。流式细胞仪检测结果显示:加入rAAV-bFGF基因转染组培养液上清的S期IPE细胞为(21.19±1.93)%,与对照组(16.47±2.37)%相比,t=-3.448,P<0.05。结论:转染rAAV-bFGF的IPE细胞分泌的bFGF具有生物活性,这为rAAV-bFGF转染IPE细胞移植治疗视网膜色素变性提供了理论支持。Aim： To measure the mitogenesia and S-cycle after iris pigment epithelial cells（IPE） infected by recombinant adeno-associated virus （rAAV）-mediated gene transfer of basic fibroblast growth factor（bFGF）. Methods：Medium of gene engineering cells culture fluid and conventional cells culture fluid were added to IPE cells cultured in vitro. The mitogenesia and cell cycle were evaluated by MTT and flow cytometer. Results： MTT showed that optical density （OD） of experiment group was 0. 338 ±0.047,and OD of control group was0.277 ±0.064（t=3.745,P〈0.05）. The percentage of S-cycle in IPE cells cultured by medium from transduced cells was （21. 19 ± 1.93 ）% ,and that in IPE cells cultured by medium from untransduced cells was （ 16.47± 2.37 ） % （ t = 3. 448, P 〈 0.05 ）. Conclusion ： IPE cells trans fected by rAAV-bFGF are able to secret bioactive bFGF protein. It is possible for the therapy of retina pigmentosa to combine rAAVbFGF gene therapy with IPE transplantation.

关 键 词：碱性成纤维细胞生长因子 基因转染 虹膜色素上皮细胞 细胞增殖 细胞周期 

分 类 号：R773.1[医药卫生—眼科]