甜菜碱对脱氮假单胞杆菌发酵产VB_(12)的影响  

Effect of betaine on the fermentation of vitamin B_(12) by Pseudomonas denitrificans

作　　者：王振国[1] 曹云峰[1] 陈学军[1] 李永亮[1] 李昆太[2] 张嗣良[2] 

机构地区：[1]石药集团河北华荣制药有限公司,河北石家庄050041 [2]华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心,上海200237

出　　处：《中国医药导报》2008年第26期12-15,共4页China Medical Herald

摘　　要：通过研究不同甲基供体(甜菜碱和氯化胆碱)对脱氮假单胞杆菌发酵产VB12的影响,结果表明甜菜碱能显著促进VB12的生物合成;在此基础上考察了发酵培养基中甜菜碱浓度对VB12合成的影响,结果表明15g/L的甜菜碱下VB12的合成量最高;进一步研究了甜菜碱对脱氮假单胞杆菌发酵过程的影响,结果表明发酵液中一定浓度的甜菜碱可以显著提高δ-氨基乙酰丙酸的合成量,从而促进VB12的合成。The effect of different methyl-group donors (betaine and choline chloride) on vitamin B12 production in shake flasks with Pseudomonas denitrificans was studied, and the results demonstrated that the addition of betaine to the fermentation medium could greatly stimulate biosynthesis of vitamin B12. Betaine concentration in fermentation medium was further investigated in shake flask, as a result, 15 g/L of betaine was the most beneficial for vitamin B12 synthesis. Moreover, the effect of betaine on the fermentation of P. denitrificans was studied, it was found that the δ-aminolevulinic acid (ALA) biosynthesis could be significantly improved when betaine was kept at certain concentration in the broth, thus the biosynthesis of vitamin B12 was greatly increased.

关 键 词：甜菜碱 菌体生长 VB12合成 Δ-氨基乙酰丙酸 脱氮假单胞杆菌 

分 类 号：R977.2[医药卫生—药品]