90例病毒性胃肠炎患者粪便中诺如病毒检出情况分析  

Nrovirus infection among 90 patients with viral gastroenteritis

作　　者：高志勇[1] 吕虹[2] 黄芳[1] 吴晓娜[1] 严寒秋[1] 刘园[1] 刘桂荣[1] 李洁[1] 窦相锋[1] 王全意[1] 庄辉[3] 

机构地区：[1]北京市疾病预防控制中心传染病地方病控制所 [2]首都医科大学附属天坛医院 [3]北京大学医学部

出　　处：《中国病原生物学杂志》2008年第8期564-566,573,共4页Journal of Pathogen Biology

基　　金：国家973项目(No2005CB523104)

摘　　要：目的了解病毒性胃肠炎患者中诺如病毒感染情况。方法收集北京市2007年2月5～17日共计90例病毒性胃肠炎患者的粪便标本,应用逆转录聚合酶链反应法(RT-PCR)和酶联免疫吸附试验(ELISA)检测粪便中诺如病毒核酸或抗原,并对RT-PCR阳性标本的PCR产物进行克隆测序。结果90例病毒性胃肠炎患者的粪便标本中,35例(38.89%)RT-PCR为诺如病毒核酸阳性,序列分析结果显示,诺如病毒GⅡ型34例(97.14%),GⅠ型1例(2.86%);90例中,39例(43.33%)为ELISA检测诺如病毒抗原阳性。以RT-PCR检测结果作为金标准进行比较,ELISA的灵敏度和特异度分别为97.14%(34/35)和90.91%(50/55)。结论诺如病毒是病毒性胃肠炎的主要病原,以GⅡ型流行为主。ELISA快速、简便,其灵敏度和特异度较高,可作为诺如病毒感染的初筛试验。Objective To investigate norovirus infection among patients with viral gastroenteritis.Methods From 5 to17 February 2007,the fecal specimens were collected from 90 sporadic cases of acute viral gastroenteritis in Beijing.Norovirus RNA and antigens were detected by a reverse transcription polymerase chain reaction（RT-PCR） and an enzyme linked immunosorbent assay（ELISA） respectively,and then the PCR products were cloned and sequenced.Results Of 90 patients with viral gastroenteritis,35（38.89%） were positive for norovirus RNA by RT-PCR,and 39（43.33%） were positive for norovirus antigens by ELISA.Sequence analysis showed that 34 strains were norovirus GⅡ type and 1 was GⅠ type.Compared with RT-PCR,the sensitivity and specificity of ELISA were 97.14%（34/35） and 90.91%（50/55） respectively.Conclusion Norovirus is the major cause of viral gastroenteritis.and GⅡ is the most prevalent genotype.ELISA is rapid,easy to perform,and may be used for preliminary screening of norovirus infection.

关 键 词：诺如病毒 病毒性胃肠炎 逆转录聚合酶链反应法(RT-PCR) 酶联免疫吸附试验(ELISA) 

分 类 号：R373.2[医药卫生—病原生物学]