荧光动力学的Laplace分析方法  

A LAPLACE TRANSFORM METHOD FOR ANALYSIS OF FLUORESCENCE DYNAMICS

作　　者：郑锡光[1] 汪河洲[1] 余振新[1] 高兆兰[1] 

机构地区：[1]中山大学激光与光谱学研究所

出　　处：《光谱学与光谱分析》1997年第6期1-6,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金;广东省自然科学基金

摘　　要：本文发展荧光动力学多指数过程的一种分析方法，即Ｌａｐｌａｃｅ变换方法，提出动力学谱函数概念，并通过计算动力学谱函数直接得到动力学过程的各参数。该方法应用于Ｒ－藻红蛋白β亚基荧光动力学的分析。A Laplace transform formula is developed for analysis of a multi exponential fluorescence dynamic process A dynamic spectrum function is introduced,calculation of which directly yields parameters of the corresponding dynamic process The formula is applied to analyze fluorescence dynamics of β subunit from R phycoerythrin It is demonstrated that electronic excited states of the biomolecular system exhibit two exponential decay processes,the faster of which relates to excitation equilibrium among chromophores, and the slower to radiative relaxation of the chromophores

关 键 词：荧光动力学 多指数过程 LAPLACE变换 激光光谱学 

分 类 号：O657.38[理学—分析化学] O643.1[理学—化学]