微型染色体维持蛋白2和Fas相关磷酸酯酶1与乳腺癌增殖和凋亡的关系  

Correlation of MCM2 and FAP-1 with proliferation and apoptosis in breast cancer

作　　者：程甜甜[1] 谢伟[1] 冯茂辉[1] 吴红艳[1] 谢辉[1] 戴凤姣[1] 龚玲玲[1] 汪付兵[1] 周云峰[1] 

机构地区：[1]武汉大学中南医院肿瘤外科,430071

出　　处：《中华实验外科杂志》2008年第9期1152-1154,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金资助项月（2004ABA159）;湖北省科技攻关资助项目（2005AA304B05、2005AA304B04）

摘　　要：目的探讨微型染色体维持蛋白2（MCM2）和Fas相关磷酸酯酶（FAP-1）的表达与乳腺癌增殖和凋亡的关系。方法采用SP法检测60例乳腺癌及15例乳腺良性疾病中MCM2及FAP-1蛋白的表达水平，10例正常乳腺组织为对照组。结果10例正常乳腺上皮、15例乳腺良性疾病、60例乳腺癌MCM2的阳性标记指数（LI）分别为6．6±1．1、14．7±3．2、44．7±4．3，各组间差异有统计学意义（P〈0．01）；MCM2的阳性表达在乳腺癌TNM分期、不同组织学分级（Ⅰ、Ⅱ、Ⅲ）及淋巴结转移之间差异有统计学意义，呈升高趋势（P〈0．05）；MCM2的LI均高于Ki-67的LI，MCM2和Ki-67在乳腺癌中的表达呈平行关系（P〈0．01）。FAP-1的阳性表达率与乳腺癌的临床分期、病理分级及淋巴结转移性明显相关（P〈0．05）；FAP-1在乳腺癌组织中的表达均显著高于正常组织和良性肿瘤。结论MCM2、FAP-1在乳腺癌组织中的表达与乳腺癌的发生发展有密切联系，MCM2可能是更理想的细胞增殖标记物，FAP-1可能具有促进乳腺癌细胞增殖的作用。Objective To investigate the relationship between the expression of MCM2 and FAP- 1 with proliferation and apoptosis in breast cancer. Methods Immunohistochemical SP method was employed to detect the expression of MCM2 and FAP-1 in benign diseases （15 cases）, breast cancers （60 cases） and the normal controls （10 cases）. In addition, clinicopathological data of them were analyzed. Resuits MCM2 labelling index increased with breast carcinogenesis,and it is higher than the labelling index of ki-67. FAP-1 increased in malignant tissue than that in benign and border line tumor tissues. Conclusion Proliferation and anti-apoptotic gene were associated with breast histopathological grade, abnormal cell proliferation and antiapoptosis are significant factors in breast cancers＇ development.

关 键 词：乳腺癌 微型染色体维持蛋白2 FAS相关磷酸酯酶-1 增殖 脱噬作用 

分 类 号：R737.9[医药卫生—肿瘤]