p55PIK N末端24个氨基酸抑制甲状腺癌细胞增殖的研究  

The study of the N-terminal 24 amino acids of the p55PIK inhibiting the proliferation of thyroid carcinoma cells

作　　者：黄焕军[1] 王桂华[2] 罗学来[2] 李小兰[2] 陶德定[2] 胡俊波[2] 龚建平[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化科,武汉430030 [2]华中科技大学同济医学院附属同济医院胃肠外科中心,武汉430030

出　　处：《中华实验外科杂志》2008年第9期1158-1160,共3页Chinese Journal of Experimental Surgery

基　　金：国家“973”计划资助项目（2002CB513100-2）

摘　　要：目的观察P55PIK的N端的24个氨基酸抑制甲状腺癌细胞增殖的作用。方法构建含有P55PIK-N端24个氨基酸的腺病毒载体Ad-N24p55PIK-GFP及对照病毒Ad-GFP，以其感染甲状腺癌FTC236细胞，流式细胞仪检测细胞周期进程，应用BrdU掺入的方法检测其对细胞DNA合成的影响，通过建立体内肿瘤裸鼠移植瘤模型，进一步证实Ad-N24p55PIK-GFP的抗肿瘤作用。结果对照腺病毒Ad-GFP感染的细胞中G0／G1期细胞为（69．83±3．50）％，S期和G2／M期细胞数分别为（18．56±3．10）％和（11．61±2．90）％，而带有插入目的基因的腺病毒Ad-N24p55PIK-GFP感染后的细胞G0／G1期细胞减少为（45．81±2．10）％，S期和G2／M期细胞增加至（30．12±2．60）％和（24.07±2．90）％，BrdU掺入显示BrdU阳性的细胞数由（21．2±3．0）％降至（8．1±2．2）％，FTC236细胞裸鼠移植瘤模型局部应用Ad-N24p55PIK-GFP后肿瘤生长显著减慢。结论在FTC236细胞中，过表达p55PIK的N末端的24个氨基酸可有效阻滞细胞周期进程，抑制细胞DNA合成，体外有效抑制甲状腺癌裸鼠移植瘤模型的肿瘤生长。Objective To elucidate the role of the N-terminal 24 amino acids of the p55PIK inhibiting the proliferation of thyroid carcinoma cells. Methods The adenovirus vector Ad-N24p55PIK-GFP containing the N-terminal 24 amino acids of the p55PIK and the control adenovirus Ad-GFP were constructed,and transfected into the thyroid cancer cell line FTC236. The cell cycle progression was detected by flow cytometer and the DNA synthesis was analyzed by BrdU/PI medthod. By using the nude mice model of FTC236 xenograft tumor, the inhibitory effects of Ad-N24p55PIK-GFP against the tumor were examined. Results In Ad-GFP group,the number of cells in G0/G1 phases was （69.83±3.50）% ,and that in the S and G2/M phases was （ 18.56±3.10） % and （ 11.61±2.90） % respectively, but in Ad-N24 p55PIK- GFP group,the number of cells in G0/G1 phases was （45.81 ± 2.10）% , and that in the S and G2/M phases cells was （ 30.12±2.6 ） % and （ 24.07±2.90） % respectively. The DNA synthesis was decreased from （21.2±3.0）% in the Ad-GFP group to （8.1±2.2）% in the Ad-N24 p55PIK-GFP group. In the nude mice model of FTC236 xenograft tumor, after local administration of the Ad-N24p55PIK-GFP, the tumor growth was significantly slowed down. Conclusion In thyroid cancer cell line FTC236 ,over-expression of the N-terminal 24 amino acids of the p55PIK can induce the cell cycle arrest,inhibit the DNA synthesis and suppress the tumor growth in nude mice model of FTC236 xenograft tumor.

关 键 词：肿瘤 腺病毒 BRDU 磷酸肌醇-3-激酶 

分 类 号：R736.1[医药卫生—肿瘤]