CSF延长慢性粒细胞白血病病理克隆体外培养存活期限作用的研究  

Colony Stimulating Factor May Prolong Survival Time of Malignant Cells of Chronic Myelogenous Leukemia in Vitro

作　　者：徐娟[1] 孙雪静[1] 杨立[1] 田丁[1] 

机构地区：[1]首都医科大学宣武医院血液科

出　　处：《首都医科大学学报》1997年第3期235-237,共3页Journal of Capital Medical University

摘　　要：为观察集落刺激因子（ＣＳＦ）对慢性粒细胞白血病（ＣＭＬ）病理克隆增殖的影响，采用单抗免疫磁珠二步分离法，从ＣＭＬ患者骨髓标本分离出带有恶性克隆标记的ＣＤ３４＋ＨＬＡＤＲ＋细胞，并在体外观察干细胞因子（ＳＣＦ）、白细胞介素３（ＩＬ３）和促红细胞生成素（Ｅｐｏ）联合应用对细胞增殖的影响。结果：ＣＳＦ存在体系与无ＣＳＦ体系相比ＣＭＬ病理克隆消失延迟１４ｄ（Ｐ＜０．０５）。结果提示：ＣＳＦ具有延长ＣＭＬ病理细胞在体外培养存活期限的作用。In order to observe the effect of colony stimulating factor on proliferation of malignant cells of chronic myelogenous leukemia (CML) CD34 +HLA DR + cells which contain ph 1 chromosome and BCR/ABL rearrangement were separated from 8 CML bone marrow sample by two step MoAb magnetical microbeads separating method,and the effect of colony stimulating factor(SCF IL 3 and Epo)on proliferation of this kind of cells were observed in vitro. The results indicated that elimination of malignant cells was delayed for 14 d in colony stimulating factor liquid culture as compared with liquid culture without colony stimulating factor( P <0.05).This observation indicates that colony stimulating factor may prolong the survival time of malignant cells of CML in vitro.

关 键 词：白血病 体外培养 集落刺激因子 CML 病理 克隆 

分 类 号：R733.720.2[医药卫生—肿瘤]