蒜氨酸酶的分离纯化条件分析  

Analysis on the Separation and Purification Condition of Alliinase

作　　者：葛艳辉[1] 赵俊英[1] 闵笛[1] 冯炘[1] 

机构地区：[1]天津理工大学环境科学与安全工程学院

出　　处：《湖北农业科学》2008年第8期945-947,共3页Hubei Agricultural Sciences

基　　金：天津市自然科学基金项目(043611111);天津市高等学校科技发展基金项目(20050901)

摘　　要：采用(NH4)2SO4盐析、Sephadex G-200凝胶过滤对蒜氨酸酶进行了部分纯化。结果表明,蒜氨酸酶的含量和酶活性在(NH4)2SO4为30%～50%时较高,40%时蒜氨酸酶活性最高;经Sephadex G-200凝胶过滤获得的酶液纯化倍数为34.45,回收率为1.72%。Alliinase was purified with （NH4）2SO4 and Sephadex G-200. The activity and content of alliinase were high while salting out with 30%-50% （NH4）2SO4,and that was the highest with 40% （NH4）2SO4. The fold of purified alliinase was 34.45 with Sephadex G-200 Gel filtration and recovery rate of that was 1.72%.

关 键 词：蒜氨酸酶 大蒜 分离 纯化 

分 类 号：Q503[生物学—生物化学]