颅内注射Aβ1-42单抗对阿尔茨海默病大鼠模型疗效的实验观察  

Curative effect observation of Aβ1-42 monoclonal antibody cerebral injection in Alzheimer's disease rat

作　　者：葛维明[1] 刘俊恒[1] 李爱民[2] 潘世扬[3] 李升[4] 张家明[5] 

机构地区：[1]江苏省省级机关医院,江苏南京210024 [2]连云港市第一人民医院,江苏连云港222000 [3]江苏省人民医院,江苏南京210029 [4]东南大学医学院,江苏南京210009 [5]江苏省中医药研究院,江苏南京210028

出　　处：《江苏预防医学》2008年第3期5-8,共4页Jiangsu Journal of Preventive Medicine

基　　金：江苏省社会发展基金资助项目(BS-2003051)

摘　　要：目的:观察淀粉样蛋白(Aβ)1-42诱导的阿尔茨海默病(AD)大鼠模型经颅内注射Aβ1-42单克隆抗体(mAb)后其行为及脑组织病理的改变。方法:利用Aβ1-42蛋白片段诱导建立AD大鼠模型后,侧脑室内注入Aβ1-42 mAb,记录Morris水迷宫试验逃生时间作为行为学指标,同时取脑组织作切片染色,观察其病理改变。结果:AD大鼠模型颅内注射Aβ1-42 mAb后,水迷宫试验逃生时间明显缩短,表明其学习记忆能力明显改善;病理切片染色观察到脑组织内Aβ沉积斑块消失。结论:颅内注射Aβ1-42 mAb对AD动物模型有明显疗效。Objective： To observe the behavior and brain tissue variation after using amyloid peptide（Aβ-42 monoclonal antibody （mAb） cerebral injection in Alzheimer＇ s disease（AD） rat. Methods： AD rat can be created with the induction of Aβ1-42 peptide. After the injection of Aβ1-42 mAb in right side ventricle, escaping time of rats in Morris Labyrinth can be recorded as the action marker. Meanwhile, pathology assay should be done with its brain tissue. Results： After cerebral injection of Aβ1-42 mAb, the escaping time of AD rat in Morris Labyrinth decreased remarkably. That means its learning and memory ability obviously improved. The result of brain tissue pathology assay show that the Aβblot disappeared. Conclusion： Aβ1-42 mAb cerebral injection do have an obvious curative effect on AD rat.

关 键 词：阿尔茨海默病 淀粉样蛋白 单克隆抗体 大鼠 

分 类 号：R-33[医药卫生]