姜黄素对人食管癌EC9706细胞凋亡的诱导作用  

Apoptosis of Human Esophageal Carcinoma Cell Line EC9706 Induced by Curcumin

作　　者：陈兰英[1] 杨海波[2] 李祺福[2] 宋建晔[2] 荆光军[2] 

机构地区：[1]平顶山工学院,河南平顶山467044 [2]教育部细胞生物学与肿瘤细胞工程重点实验室厦门大学生命科学学院,福建厦门361005

出　　处：《现代生物医学进展》2008年第9期1601-1604,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30470877)

摘　　要：目的:应用姜黄素处理人食管癌EC9706细胞,研究姜黄素对人食管癌EC9706细胞凋亡的诱导作用。方法:应用细胞计数、流式细胞仪、琼脂糖凝胶电泳、Hoechst染色、H.E染色和透射电镜检测经姜黄素诱导处理后人食管癌EC9706细胞的凋亡。结果:经姜黄素诱导处理后,人食管癌EC9706细胞生长抑制率达69.9%;细胞周期检测出现亚二倍体(亚G1期)细胞峰值,细胞凋亡率达23%;琼脂糖凝胶电泳显示出细胞凋亡典型的180-200 bp及其倍体的DNA"梯状"条带;Hoechst染色显示细胞核内出现浓染致密的固缩形态或颗粒状荧光;光镜和电镜下可见典型的细胞凋亡特征:细胞体积缩小,染色体凝集,可见有成群或单独存在的凋亡细胞,电镜下可见凋亡小体存在。结论:姜黄素能够有效诱导人食管癌EC9706细胞的凋亡,从而进一步为食管癌等恶性肿瘤疾病的治疗和凋亡机理的研究提供重要基础和科学依据.Objective： To study the apoptosis effects of the human esophageal carcinoma cell line EC9706 cells induced by curcumin （Cur）. Methods： The apoptosis effects of EC9706 cells induced by curcumin were detected by cell count, flow cytometry analysis, light microscope and electron microscope. Results： After treated with curcumin, the proliferation of EC9706 cells was inhibited, and the inhibitory rate was 69.9%. The results of flow cytometry analysis showed that curcumin could induce the emergence of the phase ofapoptosis, and the rate was 23%. Agarose gel electrophoresis revealed that cell DNA fragment exhibited characteristic ＂DNA ladder＂. Cell nucleus concentrated and appeared granular fluorescence by Hoechst33258 staining. Light microscope and electron microscope showed that the morphology of the cells treated with curcumin appeared shrinked, cell nucleus concemtrated, chromatin agglutinated, mitochondria swelled, and apoptosis body formed. Conclusion： This study suggested that curcumin could induce apoptosis of the human esophageal carcinoma cell line EC9706 effectively, and provided important foundation and research proofs to study more about the therapy of esophageal carcinoma, the malignant tumor and apoptosis mechanisms.

关 键 词：姜黄素 食管癌细胞 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤] R979.1[医药卫生—临床医学]