环糊精修饰中孔分子筛SBA15作为手性色谱固定相及其应用  

Application of β-Cyclodextrin Modified Mseoporous Spherical Particles of Mesoporous Silicate as the Substrate for Chiral High Performance Liquid Chromatography

作　　者：陈胜文[1] 邴乃慈[1] 李庆华[1] 

机构地区：[1]上海第二工业大学城市建设与环境工程学院,上海201209

出　　处：《分析化学》2008年第9期1249-1252,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.50503011);上海市教委基金(No.06RZ001);上海市"浦江人才计划"(No.05PG14501);上海市重点学科(No.P1701)资助项目

摘　　要：采用聚环氧乙烯醚-聚环氧丙烯醚-聚环氧乙烯醚(P123)为模板,正硅酸乙酯为硅源,在酸性介质中采用静置法合成出球形Φ4.3μm介孔氧化硅SBA15,通过γ-(2,3-环氧丙氧)丙基三甲氧基硅烷(KH560)在无水条件下将β-环糊精键合到SBA15表面和孔道内部。利用X射线衍射(XRD)、比表面积测定仪、红外光谱(FT-IR)、扫描电镜(SEM)和差显量热仪进行了表征。该材料湿法装填成色谱柱后成功分离了手性药物布洛芬和Dns-Phe对映体,其分离度分为别为2.03和1.04,说明其在色谱填料和手性分离上都具有广泛的应用前景。Spherical particles of mesoporous silicate （SBA15） with average pore size of 3.5 nm and particle size of 4.3 μm have been synthesized. Puronics P123 （EO20POToEO20） and cetyhrimethylammonium bromide （CTMAB） were used as surfactant and co-surfactant. β-Cyclodextrin was grafted within the channel of meso- porous silica SBA15 by means of KH560. SBA15 was characterized by XRD, FT-IR, SEM, Micromeritics ASAP and thermogravimetry-differential scanning catorimetry （TC-DSC）. The functionalized SBA15 was packed in a steel column and applied in chiral HPLC to separate chiral chemicals. Results show that Dns-Phe and chiral drugs Ibuprofen were successfully separated on the column packed with SBA15 fuctionalized with β-cyclodextrin.

关 键 词：中孔分子筛 聚环氧乙烯醚-聚环氧丙烯醚-聚环氧乙烯醚 γ-(2 3-环氧丙氧)丙基三甲氧基硅烷 Β-环糊精 手性分离 固定相 

分 类 号：O657.7[理学—分析化学]