半胱氨酸对高效液相色谱法测定多西紫杉醇白蛋白纳米粒含量的影响  

High Performance Liquid Chromatographic Determination of Docetaxel in Human Serum Albumin Nanoparticles by Addition of Cysteine

作　　者：卢懿[1] 邓意辉[1] 徐缓[1] 石莉[1] 董晓辉[1] 李乐[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《分析化学》2008年第9期1265-1268,共4页Chinese Journal of Analytical Chemistry

摘　　要：建立多西紫杉醇(docetaxel,DOC)人血清白蛋白纳米粒(human serum album in nanoparticles,HSA-NP)中药物含量测定的方法。利用半胱氨酸(cysteine,Cys)还原人血清白蛋白中交联的二硫键,释放HSA-NP中包裹的药物,再利用HPLC测定药物含量。结果表明,在本实验条件下Cys及制剂中辅料对DOC的含量测定无干扰。1μg/LCys、37℃孵化30min,最后用乙腈沉淀蛋白的方法可以完全测定出制剂中DOC含量。A method was established for the determination of docetaxel （DOC） in human serum albumin （HSA） nanoparticles by the addition of cysteine. The effect of cysteine （the disulphide bond breaker） on HSA disulphide bonds was monitored by the detection on the content of DOC from HSA-NP using HPLC. The measurements were performed on addition of 1 ~g/L free cysteine to the samples, the content assay of DOC were carried out after an incubation period of 30 min at 37 ~ under continuous shaking. The results showed that the addition of cysteine significantly increased the release of DOC from HSA-NP, and the determination results of DOC were 10% higher than that of acetonitrile.

关 键 词：半胱氨酸 二硫键 人血清白蛋白纳米粒 高效液相色谱 

分 类 号：R927[医药卫生—药学]