MDR1及MDR3基因沉默对紫杉醇耐药细胞株A2780/Taxol凋亡能力的影响  

The Effect of the Multi-drug Resistance Gene MDR1 and MDR3 Silencing on the Apoptosis of Ovary Cancer Cells A2780/Taxol

作　　者：高瑞[1] 肖兰[1] 王泽华[1] 

机构地区：[1]华中科技大学附属协和医院,湖北武汉430022

出　　处：《实用妇产科杂志》2008年第8期471-474,共4页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:探讨采用RNA干扰技术沉默多药耐药基因MDR1及MDR3后对卵巢癌紫杉醇耐药细胞株A2780/Taxol凋亡能力的影响。方法:将MDR1和MDR3的siRNA重组质粒转染A2780/Taxol,通过MTT法、流式细胞术、免疫荧光法及Western blot方法分别检测细胞对紫杉醇的敏感性、细胞周期和凋亡率、细胞中caspase-3的活性及MDR编码的P-gp蛋白的表达的变化。结果:转染SiRNAs重组质粒后,A2780/Taxol对紫杉醇敏感性的相对逆转率分别为64.3%及53.9%,细胞凋亡率由1.59%分别增加到9.43%和8.66%,细胞中caspase-3活性增强,P-gp蛋白含量明显减少,与对照组比差异有统计学意义(P<0.05)。结论:MDR1和MDR3的siRNA质粒可通过诱导卵巢癌紫杉醇耐药细胞A2780/Taxol凋亡,恢复其对紫杉醇的敏感性。Objective：To investigate cell apoptosis by silencing MDR1 and MDR3 gene on Paclitaxel resistant A2780 /Taxol cell line. Methods：siRNA plasmids targeting MDR1 and MDR3 were transfected to A2780/Taxol cell. MTT assay, flow cytometry, immunofluorescence, and Westem blot were respectively used to detect the cell sensitivity to Paclitaxel, apoptosis and cell cycle, activity of caspase-3, expression of P-gp. Results：After treatment with MDR1 and MDR3 siRNA plasmid vectors, the relative reversal efficiency of A2780/Taxol cells to Paclitaxel for two siRNA were 64.3 % and 53.9%, respectively; the apoptosis rates were increased from 1.59% to 9.43%, and 8.66% ; the activity of caspase-3 was increased and the expression of P-gp protein were significantly descended, there were signifcantly difference when compared with control group （ P 〈 0.05）. Conclusions. siRNA MDR1 and siRNA MDR3 transfection may improve sensitivity of A2780/Taxol cells to Paclitaxel by inducing apoptosis and reverse cell resis- tance to Paclitaxel.

关 键 词：RNA干扰 多药耐药 细胞凋亡 P-GP蛋白 卵巢肿瘤 

分 类 号：R737.31[医药卫生—肿瘤]