青少年特发性脊柱侧凸患者椎体生长板中Runx2及Ⅹ型胶原表达  

Expression of Runx2 and type Ⅹ collagen in human vertebral growth plates of adolescent idiopathic scoliosis

作　　者：俞扬[1] 王守丰[1] 邱勇[1] 王斌[1] 朱泽章[1] 朱锋[1] 

机构地区：[1]南京大学医学院附属鼓楼医院脊柱外科,江苏省南京市210008

出　　处：《中国脊柱脊髓杂志》2008年第9期655-659,I0001,共6页Chinese Journal of Spine and Spinal Cord

基　　金：江苏省卫生厅科研重大项目(编号:K200610)

摘　　要：目的:观察青少年特发性脊柱侧凸(AIS)患者上、下端椎和顶椎椎体生长板凸、凹侧软骨细胞的生物活性差异,探讨其在AIS发生和发展中的作用。方法:在对12例AIS患者行胸椎侧凸前路松解手术或前路矫形手术时获取上、下终椎和顶椎椎体生长板,分成凸、凹侧2组,共72枚标本,应用免疫组织化学方法检测Runx2和Ⅹ型胶原蛋白的表达,原位杂交方法检测Runx2 mRNA表达。所有染色结果通过图像分析系统进行半定量分析。结果:AIS患者顶椎椎体生长板凸、凹两侧的Ⅹ型胶原、Runx2和Runx2 mRNA表达总量存在显著性差异(P<0.05)。顶椎椎体生长板凹侧Ⅹ型胶原的表达总量低于下终椎椎体生长板凹侧的表达总量(P<0.05)。顶椎椎体生长板凹侧Runx2的表达总量低于上、下终椎椎体生长板凹侧的表达总量(P<0.05)。顶椎椎体生长板凹侧单一软骨细胞Runx2表达量高于凸侧和上、下终椎椎体生长板凹侧单一软骨细胞的表达(P<0.05)。顶椎椎体生长板凹侧高倍视野下平均Runx2 mRNA表达总量低于上、下终椎椎体生长板的凹侧(P<0.05)。顶椎椎体生长板凸侧单一细胞Runx2 mRNA表达量低于凹侧(P<0.05)。顶椎椎体生长板凹侧高倍视野下平均Ⅹ型胶原阳性细胞密度和Runx2阳性细胞密度低于凸侧和上、下终椎椎体生长板凹侧阳性细胞密度(P<0.05)。结论:AIS患者上、下终椎和顶椎椎体生长板凸、凹侧软骨细胞存在不同的生物活性和细胞动力学,这可能是力学条件改变后的一种继发性改变,但其可能在AIS的进展中发挥重要作用。Objective：To define the possible role of Runx2 and type X collagen in the induction and progression of adolescent idiopathic scoliosis.Method：Seventy two specimens of vertebral growth plates either from convex or concave side were collected at the upper end vertebrae,apex vertebrae and lower end vertebrae of the scoliotic spine from 12 patients who underwent anterior release or fusion.Expressions of Runx2 and type X collagen were examined by immunohistochemistry.The mRNA of Runx2 was detected in the specimen for three patients by the in situ hybridization.All the results were analyzed with the image analysis system.Resuit：There were significant differences of the total expression of type X collagen,Runx2 protein and Runx2 mRNA between convex side and concave side of the apex vertebral growth plates （P〈0.05）.The total expression of type X collagen in the concave side of the lower end vertebral growth plates was higher than that in the same side of apex （P〈0.05）.The total expression of Runx2 in the concave side of the upper and lower end vertebral growth plate was higher than that in the concave side of the apex （P〈0.05）.The expression of Runx2 per cell in the concave side of the apex was higher than those in the convex side of the apex and in the concave side of the upper and lower end vertebral growth plates as well（P〈0.05）.The cell density of type X collagen or Runx2 positive chondrocytes in the concave side of apex was lower than that in the convex side and in the concave side of upper and lower end vertebra（P〈0.05）.Conclusion：The difference in expression of type X collagen and Runx2 and the difference in cell density of type X collagen and Runx2 positive chondrocytes between convex side and concave side of the vertebral growth plates may implicate that the biological activity and cell kinetics of the chondrocytes in the various regions are different,which may be the secondary changes to the mechanical environment and likely play an important role in the progression of adol

关 键 词：特发性脊柱侧凸 椎体 生长板 软骨细胞 RUNX2 X型胶原 

分 类 号：R682.3[医药卫生—骨科学] R363.2[医药卫生—外科学]