左氧氟沙星滴眼液在白内障术后人眼房水中质量浓度的定量研究  

The quantitative study on concentration of levofloxacin gutta in human aqueous humor following cataract surgery

作　　者：邱辉[1] 张劲松[1] 王庆强[1] 

机构地区：[1]中国医科大学附属第四医院眼科辽宁省高校晶状体重点实验室,沈阳110005

出　　处：《眼科研究》2008年第9期693-695,共3页Chinese Ophthalmic Research

摘　　要：目的定量分析白内障患者术前不同时间、不同频次应用左氧氟沙星滴眼液后房水中药物的质量浓度。方法随机选取白内障患者90例(90眼),均实行白内障超声乳化摘出联合人工晶状体植入术,分为A、B、C组,A组为术前1d点眼,每日4次;B组为术前1d点眼,每日6次;C组为术前3d连续点眼,每日4次。所有患者均在术前1.5h进行最后1次点眼,术中取房水0.1mL,即保存于-20℃的冰箱中。采用高效液相色谱法检测房水中的药物质量浓度,并进行组间比较。结果房水中药物质量浓度A组为(0.68±0.58)μg/mL,B组为(1.05±0.66)μg/mL,C组为(1.89±0.98)μg/mL,B组高于A组,C组高于B组及A组,差异均有统计学意义(P<0.05)。结论房水中左氧氟沙星药物质量浓度与点药时间及频次呈正相关,为临床围手术期用药提供了良好的实验依据。Objective Antibacterial eye drop is often topically utilized in clinic before ocular surgery, but the standard in the application of antibacterial eye drops topically in preoperative period has not been reported. Current study was to quantitatively analyze the concentration of levofloxacin gutta in aqueous humor after topical administration before cataract surgery. Methods Total 90 patients undergoing cataract surgery were divided into A, B, C groups randomly. The levofloxacin gutta was topically used once per 6 hours for 1 day before cataract surgery in group A,and levofloxacin gutta was used once per 4 hours for 1 day before cataract surgery in group B and once per 6 hours for 3 day before surgery in group C. The eye drops was ended before one hour of cataract surgery in all patients. 0. 1 mL of aqueous humor sample was collected from every patient during the cataract surgery and was preserved in refrigerator with the temperature at -20℃. Levofloxacin gutta level in aqueous humor was determined using a validated high-performance liquid chronlatography. Results Concentration of levofloxacin gutta in aqueous humor was（0. 68 ± 0. 58） μg/mL, （ 1.05 ± 0. 66） μg/nlL and （ 1.89 ± 0. 98 ） μg/mL in group A, group B and group C respectively, showing a significant difference among these three groups compared with group A, and the levofloxacin gutta level in aqueous humor was significant higher in group C in conlparison with group A and B. Conclusion There is a positive correlation between concentration of levofloxacin gutta in aqueous humor and administration times of levofloxacin gutta before surgery.

关 键 词：左氧氟沙星 房水 年龄相关性白内障 

分 类 号：R779.66[医药卫生—眼科] R96[医药卫生—临床医学]