载脂蛋白A5—1131T〉C基因多态性与脑卒中的关系  

Relationship between apolipoprotein A5 -1131T〉C polymorphism and strokes

作　　者：李向平[1] 赵水平[1] 聂赛[1] 肖志杰[2] 卢伟[2] 彭道泉[1] 

机构地区：[1]中南大学湘雅二医院心内科,长沙410011 [2]中南大学湘雅二医院神经内科,长沙410011

出　　处：《中华神经医学杂志》2008年第9期928-931,共4页Chinese Journal of Neuromedicine

基　　金：湖南省科技计划项目（05FJ4056）

摘　　要：目的探讨载脂蛋白（Apo）A5—1131T〉C基因多态性与脑卒中的关系。方法对327例脑卒中患者（其中脑梗死患者194例，脑出血患者133例）及311名健康对照者的ApoA5-1131T〉C基因多态性及血脂水平进行检测。结果脑梗死患者的-1131C等位基因频率明显高于对照者，差异有统计学意义（P〈0．05）；脑出血患者与对照者比较，差异没有统计学意义（P〉0．05）。在脑卒中患者中，C等位基因携带者的甘油三酯（TG）水平明显高于非C携带者，差异有统计学意义（P〈0．05），总胆固醇（TC）、高密度蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL—C）比较差异无统计学意义（P〉0．05）。Logistic回归单变量分析显示TC＋CC基因型与脑梗死风险增加相关。与脑出血无关；校正体重指数（BMI）、高血压、糖尿病和HDL-C等相关因素的影响后，C等位基因仍显示为脑梗死的独立危险因素（OR=1．932，95％CI为1．057～3．532，P=0．032）。结论脑梗死患者ApoA5-1131C等位基因携带率明显高于对照者，ApoA5-1131T〉C基因多态性对血清TG水平有影响，ApoA5-1131T〉C基因变异可能增加缺血性脑卒中的易感性。Objective To investigate the relationship between the Apolipoprotein （Apo） A5 -1131T〉C polymorphism and strokes. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and polyacrylamide gel eletrophoresis （PAGE） were used to analyze the genotypic polymorphism in 327 patients with stroke （including 194 cerebral infarction patients and 133 cerebral hemorrhage patients） and 311 healthy controls. The levels of serum lipids profiles were also measured by enzymatic methods. Results The frequency of the -1131C allele in cerebral infarction patients was significantly higher than that of the control group （44.1% vs 32.3 %0, P〈0.05）, but there was not statistical difference between cerebral hemorrhage patients and controls on the -1131C allele frequency （35.4% vs 32.3%, P〉0.05）. Compared with the noncarriers, the C carriers also had a higher triglyceride （TG） levels in stroke group [（1.45±0.77） vs （1.69±1.06） mmol/L, P〈0.05], but the total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C） and low density lipoprotein cholesterol （LDL-C） levels did not show statistical differences in various genotypes （P〉0.05）. Unadjusted Logistic regression analysis indicated that TC＋CC genotype of A5 -1131T〉C was significantly associated with the presence of cerebral infarction, but not with hemorrhage stroke. Logistic regression analysis adjusted for BMI, presence of hypertension or diabetes and HDL-C levels revealed that TC＋CC genotype was an independent risk factor for cerebral infarction （OR=1.932, 95%CI： 1.057-3.532, P=0.032）. Conclusion The ApoA5 -1131C allele frequency of the patients with cerebral infarction is significantly higher than controls. ApoA5 -1131T〉C polymorphism has a significant influence on serum TG levels. The ApoA5 - 1131T〉C variant is associated with an increased susceptibility for ischemic stroke.

关 键 词：脑卒中 多态性 限制性片段长度 载脂蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]