非甾体类抗炎药对乳腺癌细胞MDA-MB-231增殖影响的实验室研究  

The laboratory study about the proliferative effect of aspirin and celecoxib on breast cancer cells line MDA-MB-231

作　　者：闫晓枫[1] 王杨[1] 张岩[1] 

机构地区：[1]吉林大学第三临床医院血液肿瘤内科,吉林长春130021

出　　处：《中国实验诊断学》2008年第9期1089-1092,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科委资助项目(200705290)

摘　　要：目的通过研究阿司匹林、塞来昔布及两者分别联合阿那曲唑对乳腺癌细胞MDA-MB-231的抑制作用,探讨并比较阿司匹林和塞来昔布的抗肿瘤作用。方法采用MTT法检测不同浓度的阿司匹林、塞来昔布及两者分别联合阿那曲唑对乳腺癌细胞MDA-MB-231增殖的影响。结果阿司匹林、塞来昔布单药对MDA-MB-231细胞生长均有抑制作用,表现为浓度、时间依赖性,统计学差异具有统计学意义(P<0.05);不同浓度的阿司匹林、塞来昔布分别联合阿那曲唑后对MDA-MB-231细胞的抑制作用较单用阿那曲唑增强,亦表现为浓度依赖性,差别具有统计学意义(P<0.05)。结论阿司匹林和塞来昔布均有抑制乳腺癌MDA-MB-231细胞的作用,两者分别联合阿那曲唑后有协同抗肿瘤的作用。Objective The objective of the experiment was to discuss and compare the anti-tumor effect of Aspirin and celecoxib on breast eaneer cells MDA-MB-231 through investigating the effects of aspirin, eelecoxib, and whieh combined with anastrozole respectively on the growth of human breast cancer cell MDA-MB-231, Methods The inhibitory effect was detected by MTT assay, and inhibitory effect time- dependently and dose-dependently, different doses of Aspirin or eelecoxib combined with the inhibitory effect Results A decrease in cell number was observed in all of the cell line treated with aspirin and celecoxib compared with controls, which was a time-dependent and dose-dependent. A value of P 〈 0.05 was considered statistically significant. And the inhibitory effect of anastrozole combined with aspirin or celecoxib on MDA-MB-231 was more than that of anastrozole single. A value of P 〈 0.05 was considered statistically significant. Conclusion, There were the inhibitory effects of aspirin and celecoxib on the growth of human breast cancer cell MDA-MB-231 ,and that was almost identical compared with ADM.There was a synergistic effect when anastrozole combined with aspirin or celecoxib on MDA-MB-231.

关 键 词：非甾体抗炎药 阿司匹林 塞来昔布 阿那曲唑 乳腺癌 人乳腺癌细胞MDA-MB-231 

分 类 号：R737.9[医药卫生—肿瘤]