阿舒假囊酵母过量合成核黄素性能的改良  

Sludies on Making Improvements in Riboflavin Overproduction of Eremothecium ashbyii

作　　者：张星元[1] 王琴[1] 

机构地区：[1]无锡轻工大学生物工程学院

出　　处：《无锡轻工大学学报（食品与生物技术）》1997年第3期14-19,共6页Journal of Wuxi University of Light Industry

摘　　要：以６０Ｃｏ的ｒ射线为诱变剂，核黄素合成途径的中间产物的结构类似物为“筛子”，筛选了阿舒假囊酵母（Ｅｒｅｍｏｔｈｅｃｉｕｍａｓｈｂｙｉ）的四种抗性突变株：杀结核菌素抗性突变株（Ｔｕｂｒ）、２－脱氧葡萄糖抗性突变株（２－ＤＧｒ）、８－氮杂鸟嘌呤（８－ＡＧｒ）和抗残液突变株。杀结核菌素抗性突变株Ｔ３０的摇瓶核黄素产量由出发菌的２．５～２．８ｍｇ／Ｌ提高并稳定在３．５ｍｇ／Ｌ以上，经初步优化（培养基配方）后，稳定在５．４ｇ／Ｌ以上。A strain of Eremothecium ashbyii isolated was used as initial strain.Four kinds of resistant strains including tubercidin resistant (Tub r),2 deoxy D glucose resistant (2 DG r),8-azguanine resistant (8 AG r),and depleted media resistant mutants,have been screened after 60 Co r rays treatment.Among them,T30,a tubercidin resistant mutant, produced riboflavin to the concentration of 3.5 mg/L while the initial srain 2.5～2.8 mg/L both under the conditions suitable for the latter.Riboflavin yield could be stable at the level of about 5.4 mg/L by means of primary optimization.

关 键 词：阿舒假囊酵母 核黄素 生物合成 杀结核菌素 

分 类 号：TQ466.2[化学工程—制药化工] TQ926.4[轻工技术与工程—发酵工程]